Jaeger C B
Neuroscience. 1986 May;18(1):121-50. doi: 10.1016/0306-4522(86)90183-1.
Immunocytochemically labeled cells containing the enzyme aromatic L-amino acid decarboxylase were localized in the brain of rat embryos at gestational age E15-E19. Cell groups that contained aromatic L-amino acid decarboxylase but lacked either the enzyme tyrosine hydroxylase or the indolamine serotonin were referred to as "D" groups. Anatomical landmarks, cytoarchitectonic structure and histochemical staining for acetylcholinesterase were used to delineate the position of "D" groups. In the E15 embryo three "D" groups existed. The first to appear, named D1, was located in the spinal cord and had been demonstrated before. A large "D" cell cluster was found in the walls of the central forebrain deep to the hypothalamic sulcus. This group distributed dorsally in the ventral dorsal thalamic region and ventrally in the dorsal hypothalamus. The rostral-most "D" group, D14, occurred in the ventral telencephalon just medial to fibers of the nigrostriatal projection. D14 was the smallest of the early groups. In E16 and E17 embryos dorsal di- and mesencephalic "D" groups were first detected. During the course of ontogeny a considerable increase of immunoreactive cells occurred and segregation of the large central forebrain cluster into several rostrally and laterally distributed "D" groups took place. Some "D" groups that occur in the adult brain were not present in the E19 embryo. This study provides a first report of the localization of several unique cell groups in the brain of rat embryos and their appearance at different stages of gestation. It also gives further support to the notion that variations of aromatic L-amino acid decarboxylase staining intensities may be characteristic of different monoamine neurons.
免疫细胞化学标记显示，含有芳香族L-氨基酸脱羧酶的细胞定位于妊娠龄E15 - E19大鼠胚胎的大脑中。含有芳香族L-氨基酸脱羧酶但缺乏酪氨酸羟化酶或吲哚胺5-羟色胺的细胞群被称为“D”组。利用解剖学标志、细胞构筑结构和乙酰胆碱酯酶的组织化学染色来确定“D”组的位置。在E15胚胎中存在三个“D”组。最先出现的一组，命名为D1，位于脊髓，之前已有报道。在丘脑沟深处的前脑中央壁中发现了一个大的“D”细胞簇。该组背侧分布于腹侧背侧丘脑区域，腹侧分布于背侧下丘脑。最靠前的“D”组，D14，出现在腹侧端脑，正好位于黑质纹状体投射纤维的内侧。D14是早期各组中最小的一组。在E16和E17胚胎中，首次检测到背侧间脑和中脑的“D”组。在个体发育过程中，免疫反应性细胞显著增加，前脑中央大簇分离为几个向头侧和外侧分布的“D”组。一些在成年大脑中出现的“D”组在E19胚胎中并不存在。本研究首次报道了大鼠胚胎大脑中几个独特细胞群的定位及其在不同妊娠阶段的出现情况。它也进一步支持了这样一种观点，即芳香族L-氨基酸脱羧酶染色强度的变化可能是不同单胺能神经元的特征。
