Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, 34469 Istanbul, Türkiye.
The Scientific and Technological Research Council of Türkiye (TÜBİTAK), Marmara Research Center (MRC), 41470 Gebze, Türkiye.
Toxins (Basel). 2023 Jun 19;15(6):402. doi: 10.3390/toxins15060402.
is a major aflatoxin B, posing significant health concerns to humans, crops, and producer fungi. Due to the undesirable consequences of the usage of synthetic fungicides, biological control using yeasts has gained more attention. In this study, eight isolates of epiphytic yeasts belonging to sp., sp. and sp., which have been identified as antagonists, were isolated from different plants, including grapes, blueberries, hawthorns, hoşkıran, beans and grape leaf. While volatile organic compounds (VOCs) produced by DN-FY, aff. DN-MP and aff. 32-AMM reduced in vitro mycelial growth and sporulation, only VOCs produced by aff. 1-UDM were found to be effective at reducing in vitro AFB production. All yeasts reduced the mycelial growth of by 76-91%, while AFB production reduced to 1.26-10.15 ng/g and the control plates' growth was 1773 ng/g. The most effective yeast, aff. DN-HS, reduced growth and aflatoxin B production on hazelnuts. The AFB content on hazelnuts reduced to 333.01 ng/g from 536.74 ng/g. To our knowledge, this is the first report of yeasts isolated from plants being tested as potential biological control agents to reduce AFB production on hazelnuts.
是一种主要的黄曲霉毒素 B，对人类、农作物和产毒真菌都构成重大健康威胁。由于合成杀真菌剂的使用存在不良后果，利用酵母进行生物防治引起了更多关注。在这项研究中，从不同植物（包括葡萄、蓝莓、山楂、hoşkıran、豆类和葡萄叶）中分离到 8 株属于 sp.、 sp. 和 sp. 的植物附生酵母，它们已被鉴定为拮抗菌。虽然 DN-FY、aff. DN-MP 和 aff. 32-AMM 产生的挥发性有机化合物（VOCs）可降低体外菌丝生长和产孢，但只有 aff. 1-UDM 产生的 VOCs 被发现可有效降低体外 AFB 产量。所有酵母均可将 的菌丝生长抑制 76-91%，而 AFB 产量降低至 1.26-10.15ng/g，对照平板的生长量为 1773ng/g。最有效的酵母 aff. DN-HS 可降低榛子上的 生长和黄曲霉毒素 B 产量。榛子上的 AFB 含量从 536.74ng/g 降低至 333.01ng/g。据我们所知，这是首次报道从植物中分离的酵母被用作潜在的生物防治剂来降低榛子上 AFB 的产量。
