Department of Health Laboratory Technology, School of Public Health, Chongqing Medical University, Chongqing, PR China.
Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, PR China.
Biofouling. 2023 Mar-Apr;39(4):444-458. doi: 10.1080/08927014.2023.2226613. Epub 2023 Jun 27.
is known for forming bacterial biofilms that confer increased antimicrobial resistance. Combining antibiotics with antibiofilm agents is an alternative approach, but the antibiofilm ability of prodigiosin (PG), a potential antibiotic synergist, against antimicrobial-resistant (AMR) remains to be understood. The antibiofilm activity of PG against 29 clinical AMR strains was evaluated using crystal violet staining, and its synergistic effects with vancomycin (VAN) was confirmed using the checkerboard test. The viability and metabolic activity of biofilms and planktonic cells were also assessed. The results revealed that PG exhibited promising inhibitory activity against biofilm formation and synergistic activity with VAN. It effectively reduced the metabolic activity of biofilms and suppressed the production of exopolysaccharides, which might be attributed to the downregulation of biofilm-related genes such as , , and . These findings suggest that PG could be used as a preventive coating or adjuvant against biofilms in clinical settings.
被认为能形成增加抗微生物药物耐药性的细菌生物膜。将抗生素与抗生物膜剂联合使用是一种替代方法，但增生霉素（PG）作为一种有潜力的抗生素增效剂的抗生物膜能力仍有待了解。使用结晶紫染色法评估 PG 对 29 株临床抗微生物药物耐药菌株的抗生物膜活性，并通过棋盘试验证实其与万古霉素（VAN）的协同作用。还评估了生物膜和浮游细胞的活力和代谢活性。结果表明，PG 对生物膜形成具有有前景的抑制活性，并与 VAN 具有协同作用。它有效地降低了生物膜的代谢活性，并抑制了胞外多糖的产生，这可能归因于生物膜相关基因如 、 和 的下调。这些发现表明，PG 可作为临床环境中预防生物膜的涂层或佐剂。
