Ma Zhiwen, Xiao Hong, Li Hailin, Lu Xiaoling, Yan Jing, Nie Hao, Yin Qi
Department of Health Laboratory Technology, School of Public Health, Chongqing Medical University, No. 61 Daxuecheng Middle Road, Shapingba District, Chongqing 401334, China.
Pathogens. 2024 Feb 5;13(2):145. doi: 10.3390/pathogens13020145.
is known to generate bacterial biofilms that increase antibiotic resistance. With the increase of multi-drug resistance in recent years, the formulation of a new therapeutic strategy has seemed urgent. Preliminary findings show that Prodigiosin (PG), derived from chromium-resistant , exhibited efficient anti-biofilm activity against . However, its anti-biofilm activity against remains largely unexplored. The anti-biofilm activity of PG against three clinical single drug-resistant was evaluated using crystal violet staining, and the viability of biofilms and planktonic cells were also assessed. A model of chronic lung infection was constructed to test the in vivo antibiofilm activity of PG. The results showed that PG inhibited biofilm formation and effectively inhibited the production of pyocyanin and extracellular polysaccharides in vitro, as well as moderated the expression of interleukins (IL-1β, IL-6, IL-10) and tumor necrosis factor (TNF-α) in vivo, which might be attributed to the downregulation of biofilm-related genes such as , , and . These findings suggest that PG could be a potential treatment for drug-resistant and chronic biofilm infections.
已知会产生增加抗生素耐药性的细菌生物膜。近年来随着多重耐药性的增加，制定新的治疗策略显得迫在眉睫。初步研究结果表明，源自耐铬菌的灵菌红素（PG）对……表现出有效的抗生物膜活性。然而，其对……的抗生物膜活性在很大程度上仍未被探索。使用结晶紫染色评估了PG对三种临床单耐药……的抗生物膜活性，还评估了生物膜和浮游细胞的活力。构建了慢性肺部感染模型以测试PG的体内抗生物膜活性。结果表明，PG在体外抑制生物膜形成并有效抑制绿脓菌素和细胞外多糖的产生，在体内还调节白细胞介素（IL-1β、IL-6、IL-10）和肿瘤坏死因子（TNF-α）的表达，这可能归因于……等生物膜相关基因的下调。这些发现表明，PG可能是耐药……和慢性生物膜感染的一种潜在治疗方法。
