Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, No.300 Jungong Road, Yangpu Area, Shanghai, 200090, People's Republic of China.
College of Fisheries and Life Science, Shanghai Ocean University, Pudong New Area, Shanghai, 201306, People's Republic of China.
Sci Rep. 2023 Jun 27;13(1):10392. doi: 10.1038/s41598-023-37626-8.
In recent years, with the widespread use of TiO-GO nanocomposite in industry, especially in the remediation of water environments, its toxic effects on aquatic organisms have received increasing attention. As molting is extremely important for crustaceans in their growth, in this study, we cloned the full-length cDNA sequences of two key genes related to molting, nuclear hormone receptor E75 (E75) and nuclear hormone receptor HR3 (HR3), in Macrobrachium rosenbergii, examined the gene expression profile, and investigated their toxicological effects on crustacean molting through nanomaterial exposure. The amino acid sequences for E75 and HR3 were respectively determined to encode 1138 and 363 acid residues. Sequence analysis showed that both E75 and HR3 contain a HOLI domain, with the E75 of M. rosenbergii being more closely related to the E75 of Palaemon carinicauda. These two genes were expressed at the highest levels in muscle, followed by hepatopancreas. The results showed that the expressions of E75 and HR3 in hepatopancreas and muscle tissues were significantly decreased after exposure to 0.1 mg/L of TiO-GO composite nanoparticles (P < 0.05). This study will serve as a foundation for subsequent research into the evaluation of nanomaterial toxicity on crustacean species.
近年来，随着 TiO-GO 纳米复合材料在工业中的广泛应用，特别是在水环境修复方面，其对水生生物的毒性作用越来越受到关注。由于蜕皮对甲壳类动物的生长极其重要，因此本研究在罗氏沼虾中克隆了与蜕皮相关的两个关键基因，即核激素受体 E75（E75）和核激素受体 HR3（HR3）的全长 cDNA 序列，分析了基因表达谱，并通过纳米材料暴露研究了它们对甲壳动物蜕皮的毒理学影响。E75 和 HR3 的氨基酸序列分别确定为编码 1138 和 363 个酸残基。序列分析表明，E75 和 HR3 均含有 HOLI 结构域，罗氏沼虾的 E75 与 Palaemon carinicauda 的 E75 更为接近。这两个基因在肌肉中的表达水平最高，其次是肝胰腺。结果表明，暴露于 0.1mg/L 的 TiO-GO 复合纳米颗粒后，肝胰腺和肌肉组织中 E75 和 HR3 的表达水平显著降低（P<0.05）。本研究将为后续研究纳米材料对甲壳类动物的毒性评估奠定基础。
