School of Life Science, East China Normal University, Shanghai, 200241, China.
Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, 210017, China.
Chemosphere. 2021 Oct;281:130827. doi: 10.1016/j.chemosphere.2021.130827. Epub 2021 May 11.
Plastic pollution in the form of nanoplastics poses a global threat to aquatic ecosystems and the organisms inhabiting them. However, few studies have been conducted on the effects of nanoplastic exposure on reproductive development in crustaceans. In order to address this issue, juvenile oriental river prawns (Macrobrachium nipponense) were exposed to different concentrations of 75-nm polystyrene nanoplastics (0, 5, 10, 20, 40 mg/L) for 28 days. In order to study the regulation of reproduction-related genes in the presence of nanoplastics, the Wee1 protein kinase gene (Wee1) and OTU domain ubiquitin aldehyde binding protein gene (OTUB) were selected. In this study, for the first time, the full-length cDNA of Mn-Wee1 and Mn-OTUB were cloned from M. nipponense. Homologous alignments revealed that Mn-Wee1 had a highly conserved function-critical sequence, and that Mn-OTUB was more closely related to OTUB1 than OTUB2. With increasing concentration of nanoplastics, the expression of both genes increased initially, then decreased. The inhibition of expression of Wee1 and OTUB occurred in 40 mg/L group, respectively. Analysis of the data also indicated that nanoplastic exposure might have differing effects on gene expression in M. nipponense male and female reproductive organs.
纳米塑料以塑料污染的形式对水生生态系统和其中的生物构成全球性威胁。然而，关于纳米塑料暴露对甲壳类动物生殖发育影响的研究还很少。为了解决这个问题，将幼年日本沼虾（Macrobrachium nipponense）暴露于不同浓度的 75nm 聚苯乙烯纳米塑料（0、5、10、20、40mg/L）中 28 天。为了研究纳米塑料存在下与生殖相关的基因调控，选择了 Wee1 蛋白激酶基因（Wee1）和 OTU 结构域泛素醛结合蛋白基因（OTUB）。在这项研究中，首次从 M.nipponense 中克隆出 Mn-Wee1 和 Mn-OTUB 的全长 cDNA。同源比对显示 Mn-Wee1 具有高度保守的功能关键序列，Mn-OTUB 与 OTUB1 的关系比 OTUB2 更密切。随着纳米塑料浓度的增加，这两个基因的表达最初都增加，然后减少。在 40mg/L 组中，Wee1 和 OTUB 的表达均受到抑制。数据分析还表明，纳米塑料暴露可能对 M.nipponense 雄性和雌性生殖器官中的基因表达产生不同的影响。
