非洲猪瘟病毒CD2v蛋白的细胞内表位引发体液免疫和细胞免疫反应。

An Intracellular Epitope of ASFV CD2v Protein Elicits Humoral and Cellular Immune Responses.

作者信息

Lu Wenlong, Bai Yilin, Zhang Shuai, Zhao Xuyang, Jin Jiaxin, Zhu Xiaojing, Wang Rui, Wu Yanan, Zhang Angke, Zhang Gaiping, Zhuang Guoqing, Sun Aijun

机构信息

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.

International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.

出版信息

Animals (Basel). 2023 Jun 12;13(12):1967. doi: 10.3390/ani13121967.

The African swine fever virus (ASFV) causes high mortality in domestic pigs. ASFV encodes an important protein target for subunit vaccine development, CD2v, but its most effective immunological regions are not known. Herein, we generated a monoclonal antibody (mAb) named IF3 by immunizing mice against the intracellular region of the CD2v protein (CD2v-IR). 1F3 specifically recognized CD2v, which is expressed transiently in transfected Sf9 cells and also in inactivated ASFV-infected porcine alveolar macrophage (PAM) cells. The epitope recognized by 1F3 is EPSPREP, which is highly conserved in ASFV genotypes. Immunization of mice with this epitope elicited an increased IgG response, including IgG1 and IgG2a subtypes, and also increased CD8 T cells and cytokine expression. Overall, these results indicate that this epitope induces both humoral and cellular immune responses that may be used for ASFV-related subunit vaccine design and development.

非洲猪瘟病毒（ASFV）可导致家猪的高死亡率。ASFV编码一种用于亚单位疫苗开发的重要蛋白靶点CD2v，但其最有效的免疫区域尚不清楚。在此，我们通过用针对CD2v蛋白细胞内区域（CD2v-IR）的抗原免疫小鼠，产生了一种名为IF3的单克隆抗体（mAb）。1F3特异性识别CD2v，其在转染的Sf9细胞中短暂表达，也在灭活的ASFV感染的猪肺泡巨噬细胞（PAM）中表达。1F3识别的表位是EPSPREP，其在ASFV基因型中高度保守。用该表位免疫小鼠可引起IgG反应增加，包括IgG1和IgG2a亚型，还可增加CD8 T细胞和细胞因子表达。总体而言，这些结果表明该表位可诱导体液免疫和细胞免疫反应，可用于ASFV相关亚单位疫苗的设计和开发。