Ding Yanan, Xiu Huanhuan, Zhang Yanling, Ke Miaola, Lin Letao, Yan Huzheng, Hu Pan, Xiao Meigui, He Xu, Zhang Tao
Department of Otolaryngology, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou 510630, China.
Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510062, China.
Biomedicines. 2023 May 30;11(6):1581. doi: 10.3390/biomedicines11061581.
Ionizing radiation (IR) is an important treatment for nasopharyngeal carcinoma (NPC) that mainly kills tumor cells by producing large amounts of reactive oxygen species (ROS). Intracellular ROS levels affect the sensitivity of tumor cells to IR. Recently, angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin-converting enzyme (ACE) have been found to affect the intracellular levels of ROS. Therefore, we performed a health informatics assessment of ACE in the TCGA database. We explored the effect of ACE in NPC cells. We found that either knockdown of ACE or inhibition of ACE by enalaprilat could decrease ROS levels in NPC cells. Furthermore, knockdown of ACE or inhibition of ACE by enalaprilat could reduce IR-induced ROS levels. ACE knockdown or inhibition reduced IR-induced DNA damage and apoptosis. ACE overexpression increased the level of ROS in NPC cells and further increased sensitivity to IR. These findings indicate that ACE influences the effect of IR by regulating the level of ROS in NPC cells.
电离辐射（IR）是鼻咽癌（NPC）的一种重要治疗方法，主要通过产生大量活性氧（ROS）来杀死肿瘤细胞。细胞内ROS水平影响肿瘤细胞对IR的敏感性。最近，已发现血管紧张素转换酶抑制剂（ACEIs）和血管紧张素转换酶（ACE）会影响细胞内ROS水平。因此，我们在TCGA数据库中对ACE进行了健康信息学评估。我们探究了ACE在NPC细胞中的作用。我们发现，敲低ACE或用依那普利拉抑制ACE均可降低NPC细胞中的ROS水平。此外，敲低ACE或用依那普利拉抑制ACE可降低IR诱导的ROS水平。ACE敲低或抑制可减少IR诱导的DNA损伤和细胞凋亡。ACE过表达会增加NPC细胞中的ROS水平，并进一步增加对IR的敏感性。这些发现表明，ACE通过调节NPC细胞中的ROS水平来影响IR的疗效。
