González-Reguero Daniel, Robas-Mora Marina, Fernández-Pastrana Vanesa M, Probanza-Lobo Agustín, Jiménez-Gómez Pedro Antonio
Department of Pharmaceutical Science and Health, San Pablo University, CEU Universities, Ctra. Boadilla del Monte Km 5.300, 28668 Boadilla del Monte, Spain.
Biology (Basel). 2023 May 31;12(6):801. doi: 10.3390/biology12060801.
The emergence of antibiotic resistance (AR) poses a threat to the "One Health" approach. Likewise, mercury (Hg) pollution is a serious environmental and public health problem. Its ability to biomagnify through trophic levels induces numerous pathologies in humans. As well, it is known that Hg-resistance genes and AR genes are co-selected. The use of plant-growth-promoting bacteria (PGPB) can improve plant adaptation, decontamination of toxic compounds and control of AR dispersal. The cenoantibiogram, a technique that allows estimating the minimum inhibitory concentration (MIC) of a microbial community, has been postulated as a tool to effectively evaluate the evolution of a soil. The present study uses the metagenomics of gene amplicons to understand the distribution of the microbial soil community prior to bacterial inoculation, and the cenoantibiogram technique to evaluate the ability of four PGPB and their consortia to minimize antibiotic resistance in the rhizosphere of var. Orden Dorado grown in Hg-contaminated soils. Results showed that the addition of A1 strain () and its consortia with A2, B1 and B2 strains reduced the edaphic community´s MIC against cephalosporins, ertapenem and tigecycline. The metagenomic study revealed that the high MIC of non-inoculated soils could be explained by the bacteria which belong to the detected taxa,. showing a high prevalence of Proteobacteria, Cyanobacteria and Actinobacteria.
抗生素耐药性（AR）的出现对“同一健康”方法构成了威胁。同样，汞（Hg）污染是一个严重的环境和公共卫生问题。其通过营养级进行生物放大的能力会引发人类的多种病症。此外，已知汞抗性基因和抗生素耐药基因会共同被选择。使用促植物生长细菌（PGPB）可以改善植物适应性、对有毒化合物进行去污以及控制抗生素耐药性的传播。群落抗生素药敏谱，一种用于估计微生物群落最小抑菌浓度（MIC）的技术，已被假定为有效评估土壤演变的工具。本研究利用基因扩增子宏基因组学来了解细菌接种前土壤微生物群落的分布，并使用群落抗生素药敏谱技术来评估四种促植物生长细菌及其联合体在受汞污染土壤中种植的Orden Dorado品种根际中最小化抗生素耐药性的能力。结果表明，添加A1菌株（ ）及其与A2、B1和B2菌株的联合体降低了土壤群落对头孢菌素、厄他培南和替加环素的最小抑菌浓度。宏基因组学研究表明，未接种土壤的高最小抑菌浓度可以由属于检测到的分类群的细菌来解释， ，变形菌门、蓝细菌门和放线菌门的患病率较高。
