Escola de Ciências da Saúde, Universidade Salvador, Salvador 41720-200, BA, Brazil.
Laboratório de Investigação do Vínculo Genético, Centro de Diagnóstico do GACC (CDG), Grupo de Apoio à Criança com Câncer (GACC), Salvador 41250-010, BA, Brazil.
Genes (Basel). 2023 May 29;14(6):1185. doi: 10.3390/genes14061185.
Identifying DNA markers such as Short Tandem Repeats (STR) can be used to investigate genetic diversity based on levels of heterozygosity within and between populations. Allele frequencies and forensic data for STRs were obtained from a sample of 384 unrelated individuals living in Bahia, Northeastern Brazil. Thus, the present study aimed to identify the allele frequency distribution, in addition to the forensic and genetic data, of 25 STR loci in the population of Bahia. Buccal swabs or fingertip punctures were utilized to amplify and detect 25 DNA markers. The most polymorphic loci were SE33 (43), D21S11, and FGA (21). The least polymorphic were TH01 (6), TPOX, and D3S1358 (7). Forensic and statistical data were obtained through data analysis, which revealed a large genetic diversity, with an average value of 0.813 for the analyzed population. The present study was more robust than previous STR marker studies and will contribute to future research on population genetics in Brazil and worldwide. The results of this study allowed the establishment of haplotypes found in the forensic samples of Bahia State to serve as a reference in the elucidation of criminal cases and paternity tests, as well as population and evolutionary investigations.
鉴定 DNA 标记物,如短串联重复序列 (STR),可用于根据群体内部和群体之间的杂合度水平来研究遗传多样性。本研究从居住在巴西东北部巴伊亚州的 384 名无血缘关系的个体中获取了 STR 的等位基因频率和法医学数据。因此,本研究旨在鉴定巴伊亚州人群中 25 个 STR 基因座的等位基因频率分布,以及法医和遗传数据。本研究使用口腔拭子或指尖穿刺来扩增和检测 25 个 DNA 标记物。多态性最高的基因座是 SE33(43)、D21S11 和 FGA(21)。多态性最低的是 TH01(6)、TPOX 和 D3S1358(7)。通过数据分析获得了法医和统计数据,结果表明该分析人群具有较大的遗传多样性,平均值为 0.813。本研究比以前的 STR 标记物研究更为可靠,将有助于未来对巴西乃至全球人群遗传学的研究。本研究的结果使得在巴伊亚州法医样本中发现的单倍型可以作为刑事案件和亲子关系鉴定以及人群和进化研究的参考。