School of Horticulture, Anhui Agricultural University, Hefei 230036, China.
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Genes (Basel). 2023 Jun 15;14(6):1272. doi: 10.3390/genes14061272.
Leaf margin serration is a morphological characteristic in plants. The () gene plays an important role in the outgrowth of leaf teeth and enhances leaf serration via suppression of growth in the sinus. In this study, we isolated the gene from Pak-choi (B ssp. ), which contains a 1104 bp coding sequence, encoding 367 amino acid residues. Multiple sequence alignment exhibited that the gene has a typical conserved NAC domain, and phylogenetic relationship analysis showed that the protein has high identity with plants (, , and ). The tissue-specific expression analysis displayed that the gene has relatively high transcript abundance in floral organs. Meanwhile, the expression profile of was relatively higher in the '082' lines with serrate leaf margins than the '001' lines with smooth leaf margins in young leaves, roots, and hypocotyls. In addition, the transcript level of was up-regulated by IAA and GA3 treatment, especially at 1-3 h. The subcellular localization assay demonstrated that BcCUC2 was a nuclear-target protein. Furthermore, leaf serration occurred, and the number of the inflorescence stem was increased in the transgenic plants' overexpressed gene. These data illustrated that is involved in the development of leaf margin serration, lateral branches, and floral organs, contributing to further uncovering and perfecting the regulation mechanism of leaf serration in Pak-choi.
叶片边缘锯齿是植物的一种形态特征。()基因在叶片齿的生长中起着重要作用，通过抑制窦的生长来增强叶片锯齿。在这项研究中，我们从白菜（B ssp.）中分离出了该基因，该基因包含一个 1104 个碱基对的编码序列，编码 367 个氨基酸残基。多重序列比对显示，该基因具有典型的保守 NAC 结构域，系统发育关系分析表明，该蛋白与()植物具有高度的同源性（，，和）。组织特异性表达分析显示，该基因在花器官中有相对较高的转录丰度。同时，在具有锯齿状叶片边缘的 '082' 系和具有光滑叶片边缘的 '001' 系的幼叶、根和下胚轴中，的表达谱相对较高。此外，IAA 和 GA3 处理上调了的转录水平，尤其是在 1-3 小时。亚细胞定位试验表明 BcCUC2 是一种核靶向蛋白。此外，在过表达基因的转基因植物中，叶片边缘锯齿出现，花序茎的数量增加。这些数据表明，在白菜叶片边缘锯齿、侧枝和花器官的发育中起作用，有助于进一步揭示和完善白菜叶片锯齿的调控机制。
