Department of Surgery, Section of Plastic and Reconstructive Surgery, National Cheng Kung University Hospital, College of Medicine, International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan 704, Taiwan.
Department of Chemical and Materials Engineering, National Central University, Taoyuan 32001, Taiwan.
Int J Mol Sci. 2023 Jun 6;24(12):9822. doi: 10.3390/ijms24129822.
Emerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and explored as essential markers for diagnostic medicine. However, the role of exosomal miR-21 in wounds has yet to be well studied. In order to facilitate the early management of poorly healing wounds, we developed an easy-to-use, rapid, paper-based microfluidic-exosomal miR-21 extraction device to determine wound prognosis in a timely manner. We isolated and then quantitatively examined exosomal miR-21 in wound fluids from normal tissues and acute and chronic wounds. Eight improving wounds displayed lower levels of exosomal miR-21 expression after wound debridement. However, four instances of increased exosomal miR-21 expression levels were notably associated with patients with poor healing wounds despite aggressive wound debridement, indicating a predictive role of tissue exosomal miR-21 for wound outcome. Paper-based nucleic acid extraction device provides a rapid and user-friendly approach for evaluating exosomal miR-21 in wound fluids as a means of monitoring wounds. Our data suggest that tissue exosomal miR-21 is a reliable marker for determining current wound status.
新出现的证据表明，microRNAs 在伤口愈合中发挥着关键作用。先前发现 microRNA-21 (miR-21) 上调，以在伤口中发挥抗炎作用。外泌体 microRNAs 已被确定并探索为诊断医学的重要标志物。然而，外泌体 miR-21 在伤口中的作用尚未得到很好的研究。为了便于对愈合不良的伤口进行早期管理，我们开发了一种易于使用、快速、基于纸张的微流控外泌体 miR-21 提取设备，以便及时确定伤口预后。我们从正常组织和急性及慢性伤口的伤口液中分离并定量检查了外泌体 miR-21。在清创后，8 例改善的伤口中外泌体 miR-21 的表达水平较低。然而，尽管进行了积极的清创，但 4 例外泌体 miR-21 表达水平升高与愈合不良的伤口患者明显相关，表明组织外泌体 miR-21 对伤口结局具有预测作用。基于纸张的核酸提取设备为评估伤口液中外泌体 miR-21 提供了一种快速、用户友好的方法，作为监测伤口的手段。我们的数据表明，组织外泌体 miR-21 是确定当前伤口状态的可靠标志物。
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