School of Pharmaceutical Science, Sun Yat-Sen University, Guangzhou 510006, China.
Int J Mol Sci. 2023 Jun 15;24(12):10183. doi: 10.3390/ijms241210183.
In recent years, aggregation-induced emission enhancement (AIEE) molecules have shown great potential for applications in the fields of bio-detection, imaging, optoelectronic devices, and chemical sensing. Based on our previous studies, we investigated the fluorescence properties of six flavonoids and confirmed that compounds - have good aggregation-induced emission enhancement (AIEE) properties through a series of spectroscopic experiments. Compounds with AIEE properties have addressed the limitation imposed by the aggregation-caused quenching (ACQ) of classic organic dyes owing to their strong fluorescence emission and high quantum yield. Based on their excellent fluorescence properties, we evaluated their performance in the cell and we found that they could label mitochondria specifically by comparing their Pearson correlation coefficients (R) with Mito Tracker Red and Lyso-Tracker Red. This suggests their future application in mitochondrial imaging. Furthermore, studies of uptake and distribution characterization in 48 hpf zebrafish larvae revealed their potential for monitoring real-time drug behavior. The uptake of compounds by larvae varies significantly across different time cycles (between uptake and utilization in the tissue). This observation has important implications for the development of visualization techniques for pharmacokinetic processes and can enable real-time feedback. More interestingly, according to the data presented, tested compounds aggregated in the liver and intestine of 168 hpf larvae. This finding suggests that they could potentially be used for monitoring and diagnosing liver and intestinal diseases.
近年来，聚集诱导发光增强（AIEE）分子在生物检测、成像、光电设备和化学传感等领域的应用中显示出巨大的潜力。基于我们之前的研究，我们研究了六种类黄酮的荧光性质，并通过一系列光谱实验证实了化合物 - 具有良好的聚集诱导发光增强（AIEE）性质。具有 AIEE 性质的化合物解决了由于经典有机染料的聚集引起的猝灭（ACQ）而导致的限制，因为它们具有强烈的荧光发射和高量子产率。基于它们优异的荧光性质，我们评估了它们在细胞中的性能，发现它们可以通过比较与 Mito Tracker Red 和 Lyso-Tracker Red 的 Pearson 相关系数（R）来特异性标记线粒体。这表明它们未来在线粒体成像中的应用。此外，对 48 hpf 斑马鱼幼虫摄取和分布特征的研究表明，它们具有监测实时药物行为的潜力。幼虫对化合物的摄取在不同的时间周期（在组织中摄取和利用之间）有很大差异。这一观察结果对开发药代动力学过程的可视化技术具有重要意义，并能实现实时反馈。更有趣的是，根据所提供的数据，在 168 hpf 幼虫的肝脏和肠道中聚集了测试化合物。这一发现表明，它们可能潜在地用于监测和诊断肝脏和肠道疾病。
