Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2023 Jun 16;24(12):10228. doi: 10.3390/ijms241210228.
MORN proteins play a key role in the cytoskeletal structure of eukaryotes and are essential for the close arrangement of the endoplasmic reticulum and plasma membrane. A gene with nine MORN motifs (TGGT1_292120, named Tg) was identified in the genome; it was presumed to belong to the MORN protein family and to have the function of forming the cytoskeleton, which affects the survival of . However, the genetic deletion of did not noticeably affect parasite growth and virulence. Using adjacent protein labeling techniques, we identified a network of TgMORN2 interactions, which mainly included endoplasmic reticulum stress (ER stress)-related proteins. In exploring these data, we found that the pathogenicity of the KO-Tg strain was significantly reduced in the case of tunicamycin-induced ER stress. Reticulon TgRTN (TGGT1_226430) and tubulin β-Tubulin were identified as interaction proteins of TgMORN2. Collectively, TgMORN2 plays a role in ER stress, which lays a foundation for further research on the function of the MORN protein in .
MORN 蛋白在真核生物的细胞骨架结构中发挥着关键作用，对于内质网和质膜的紧密排列至关重要。在基因组中鉴定到一个具有九个 MORN 基序（TGGT1_292120，命名为 Tg）的基因；它被推测属于 MORN 蛋白家族，具有形成细胞骨架的功能，这会影响 的存活。然而， 的基因缺失并没有明显影响寄生虫的生长和毒力。使用相邻蛋白标记技术，我们鉴定了一个 TgMORN2 相互作用网络，主要包括内质网应激（ER 应激）相关蛋白。在探索这些数据时，我们发现，在衣霉素诱导的 ER 应激情况下，KO-Tg 菌株的致病性显著降低。Reticulon TgRTN（TGGT1_226430）和微管蛋白β-Tubulin 被鉴定为 TgMORN2 的相互作用蛋白。总之，TgMORN2 在内质网应激中发挥作用，为进一步研究 MORN 蛋白在 中的功能奠定了基础。
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