Departments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau, Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg, 06847 Dessau, Germany.
Division of Medicine, Department of Gastroenterology, Metabolism and Oncology, University Hospital Ruppin-Brandenburg, Brandenburg Medical School and Faculty of Health Sciences Brandenburg, 16816 Neuruppin, Germany.
Int J Mol Sci. 2023 Jun 19;24(12):10332. doi: 10.3390/ijms241210332.
The seeds of (horseradish tree) contain about 40% of one of the most stable vegetable oils (Moringa seed oil). Therefore, the effects of Moringa seed oil on human SZ95 sebocytes were investigated and were compared with other vegetable oils. Immortalized human SZ95 sebocytes were treated with Moringa seed oil, olive oil, sunflower oil, linoleic acid and oleic acid. Lipid droplets were visualized by Nile Red fluorescence, cytokine secretion via cytokine antibody array, cell viability with calcein-AM fluorescence, cell proliferation by real-time cell analysis, and fatty acids were determined by gas chromatography. Statistical analysis was performed by the Wilcoxon matched-pairs signed-rank test, the Kruskal-Wallis test and Dunn's multiple comparison test. The vegetable oils tested stimulated sebaceous lipogenesis in a concentration-dependent manner. The pattern of lipogenesis induced by Moringa seed oil and olive oil was comparable to lipogenesis stimulated by oleic acid with also similar fatty acid secretion and cell proliferation patterns. Sunflower oil induced the strongest lipogenesis among the tested oils and fatty acids. There were also differences in cytokine secretion, induced by treatment with different oils. Moringa seed oil and olive oil, but not sunflower oil, reduced the secretion of pro-inflammatory cytokines, in comparison to untreated cells, and exhibited a low n-6/n-3 index. The anti-inflammatory oleic acid detected in Moringa seed oil probably contributed to its low levels of pro-inflammatory cytokine secretion and induction of cell death. In conclusion, Moringa seed oil seems to concentrate several desired oil properties on sebocytes, such as high content level of the anti-inflammatory fatty acid oleic acid, induction of similar cell proliferation and lipogenesis patterns compared with oleic acid, lipogenesis with a low n-6/n-3 index and inhibition of secretion of pro-inflammatory cytokines. These properties characterize Moringa seed oil as an interesting nutrient and a promising ingredient in skin care products.
辣木树的种子含有约 40%的最稳定的植物油之一（辣木籽油）。因此，研究了辣木籽油对人 SZ95 皮脂细胞的影响，并与其他植物油进行了比较。用辣木籽油、橄榄油、葵花籽油、亚油酸和油酸处理永生化人 SZ95 皮脂细胞。通过尼罗红荧光可视化脂滴，通过细胞因子抗体阵列检测细胞因子分泌，通过 calcein-AM 荧光检测细胞活力，通过实时细胞分析检测细胞增殖，通过气相色谱法测定脂肪酸。采用 Wilcoxon 配对符号秩检验、Kruskal-Wallis 检验和 Dunn 多重比较检验进行统计分析。测试的植物油以浓度依赖的方式刺激皮脂生成。辣木籽油和橄榄油诱导的脂生成模式与油酸刺激的脂生成模式相似，具有相似的脂肪酸分泌和细胞增殖模式。葵花籽油在测试的油中诱导最强的脂生成。不同油处理诱导的细胞因子分泌也存在差异。与未处理细胞相比，辣木籽油和橄榄油降低了促炎细胞因子的分泌，并且 n-6/n-3 指数较低，而葵花籽油则没有。辣木籽油中检测到的抗炎性油酸可能是其促炎细胞因子分泌和诱导细胞死亡水平较低的原因。总之，辣木籽油似乎集中了几种皮脂细胞所需的油特性，例如具有抗炎性脂肪酸油酸的高含量水平、与油酸相比诱导相似的细胞增殖和脂生成模式、低 n-6/n-3 指数的脂生成以及促炎细胞因子分泌的抑制。这些特性使辣木籽油成为一种有趣的营养物质和有前途的护肤品成分。
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