Mechanics of Active Fluids and Bacterial Physics Laboratory, Department of Civil and Environmental Engineering, Western University, London, ON N6A 3K7, Canada.
Department of Health and Society, University of Toronto, Toronto, ON M1C 1A4, Canada.
Int J Mol Sci. 2023 Jun 19;24(12):10333. doi: 10.3390/ijms241210333.
is a unicellular microalga which can cause massive mortality in both wild and cultivated fish worldwide, resulting in substantial economic losses. Environmental parameters such as salinity, light, and temperature showed a significant effect on bloom initiation and the toxicity of . While in previous studies a one-factor-at-a-time (OFAT) approach was utilized, which only changes one variable at a time while keeping others constant, in the current study a more precise and effective design of experiment (DOE) approach, was used to investigate the simultaneous effect of three factors and their interactions. The study employed a central composite design (CCD) to investigate the effect of salinity, light intensity, and temperature on the toxicity, lipid, and protein production of . A yeast cell assay was developed to assess toxicity, which offers rapid and convenient cytotoxicity measurements using a lower volume of samples compared to conventional methods using the whole organism. The obtained results showed that the optimum condition for toxicity of was 25 °C, a salinity of 17.5, and a light intensity of 250 μmol photons m s. The highest amount of lipid and protein was found at 25 °C, a salinity of 30, and a light intensity of 250 μmol photons m s. Consequently, the combination of warm water mixing with lower salinity river input has the potential to enhance toxicity, which aligns with environmental reports that establish a correlation between warm summers and extensive runoff conditions that indicate the greatest concern for aquaculture facilities.
是一种单细胞微藻，它可以在全球范围内导致野生和养殖鱼类大量死亡，造成巨大的经济损失。环境参数，如盐度、光照和温度，对藻类的爆发和毒性有显著影响。虽然在以前的研究中，采用了单因素分析法（OFAT），这种方法每次只改变一个变量，而保持其他变量不变，但在目前的研究中，采用了更精确和有效的实验设计（DOE）方法，来研究三个因素及其相互作用的同时影响。该研究采用中心复合设计（CCD）来研究盐度、光照强度和温度对毒性、脂质和蛋白质生产的影响。开发了一种酵母细胞测定法来评估毒性，该方法使用较少的样品量，与使用整个生物体的传统方法相比，可以快速方便地进行细胞毒性测量。所得结果表明，毒性的最佳条件为 25°C、盐度 17.5 和光照强度 250 μmol 光子 m s。在 25°C、盐度 30 和光照强度 250 μmol 光子 m s 时，脂质和蛋白质的含量最高。因此，温水混合低盐水流入的组合可能会增强的毒性，这与环境报告一致，该报告建立了温暖的夏季和广泛的径流条件之间的相关性，表明水产养殖设施存在最大的担忧。
