Villarejo-Campos Pedro, García-Arranz Mariano, Qian Siyuan, Jiménez de Los Galanes Santos, Domínguez-Prieto Víctor, Vélez-Pinto Juan Felipe, Guijo Castellano Ismael, Jiménez-Fuertes Montiel, Guadalajara Héctor, García-Olmo Damián
Department of Surgery, Fundación Jiménez Díaz University Hospital, Avda. Reyes Católicos, 2, 28040 Madrid, Spain.
Department of Surgery, Universidad Autónoma de Madrid, C/Arzobispo Morcillo s/n, 28034 Madrid, Spain.
J Clin Med. 2023 Jun 12;12(12):4007. doi: 10.3390/jcm12124007.
Pseudomyxoma peritonei (PMP) is a rare malignant growth characterized by the production of mucin and the potential for peritoneal relapse. This study aimed to investigate the immunohistochemical and biological characteristics of mucin in patients with cellular and acellular PMP. We prospectively analyzed mucin specimens obtained from our patient cohort and described the composition and type of mucin present in each sample. A metagenomic analysis of the samples was performed to investigate the bacterial composition of the PMP microbiome. Secreted mucins 2 and 5AC and membrane-associated mucin-1 were the primary components of mucin in both cellular and acellular tumor specimens. The metagenomic study revealed a predominance of the phylum and the genus . Notably, , a species not previously reported in the human microbiome, was found to be the most abundant organism in the mucin of pseudomyxoma peritonei. Our findings suggest that the presence of MUC-2 and mucin colonization by Pseudomonas are characteristic features of both cellular and acellular disease. These results may have significant implications for the diagnosis and treatment of this rare entity.
腹膜假黏液瘤（PMP）是一种罕见的恶性肿瘤，其特征是产生黏蛋白并具有腹膜复发的可能性。本研究旨在调查细胞性和无细胞性PMP患者中黏蛋白的免疫组织化学和生物学特性。我们对从患者队列中获取的黏蛋白标本进行了前瞻性分析，并描述了每个样本中存在的黏蛋白的组成和类型。对样本进行宏基因组分析以研究PMP微生物群的细菌组成。分泌型黏蛋白2和5AC以及膜相关黏蛋白-1是细胞性和无细胞性肿瘤标本中黏蛋白的主要成分。宏基因组研究显示 门和 属占优势。值得注意的是，在人类微生物群中以前未报告过的 种被发现是腹膜假黏液瘤黏蛋白中最丰富的生物体。我们的研究结果表明，MUC-2的存在和假单胞菌引起的黏蛋白定植是细胞性和无细胞性疾病的特征。这些结果可能对这种罕见疾病的诊断和治疗具有重要意义。
