Rocchetti Maria Teresa, Bizzoca Davide, Moretti Lorenzo, Ragni Enrico, Moretti Francesco Luca, Vicenti Giovanni, Solarino Giuseppe, Rizzello Alessandro, Petruzzella Vittoria, Palese Luigi Leonardo, Scacco Salvatore, Banfi Giuseppe, Moretti Biagio, Gnoni Antonio
Department of Clinical and Experimental Medicine, University of Foggia, Via Pinto 1, 71122 Foggia, Italy.
Orthopaedics Unit-UOSD Vertebral Surgery, DAI Neuroscience, Sense Organs and Locomotor System, AOU Consorziale Policlinico, 70124 Bari, Italy.
J Pers Med. 2023 May 30;13(6):916. doi: 10.3390/jpm13060916.
The identification of synovial fluid (SF) biomarkers that could anticipate the diagnosis of osteoarthritis (OA) is gaining increasing importance in orthopaedic clinical practice. This controlled trial aims to assess the differences between the SF proteome of patients affected by severe OA undergoing Total Knee Replacement (TKR) compared to control subjects (i.e., subjects younger than 35, undergoing knee arthroscopy for acute meniscus injury).
The synovial samples were collected from patients with Kellgren Lawrence grade 3 and 4 knee osteoarthritis undergoing THR (study group) and young patients with meniscal tears and no OA signs undergoing arthroscopic surgery (control group). The samples were processed and analyzed following the protocol defined in our previous study. All of the patients underwent clinical evaluation using the International Knee Documentation Committee (IKDC) subjective knee evaluation (main outcome), Knee Society Clinical Rating System (KSS), Knee injury and Osteoarthritis Outcome Score (KOOS), and Visual Analogue Scale (VAS) for pain. The drugs' assumptions and comorbidities were recorded. All patients underwent preoperative serial blood tests, including complete blood count and C-Reactive Protein (CRP).
The synovial samples' analysis showed a significantly different fibrinogen beta chain (FBG) and alpha-enolase 1 (ENO1) concentration in OA compared to the control samples. A significant correlation between clinical scores, FBG, and ENO1 concentration was observed in osteoarthritic patients.
Synovial fluid FBG and ENO1 concentrations are significantly different in patients affected by knee OA compared with non-OA subjects.
在骨科临床实践中，识别能够预测骨关节炎（OA）诊断的滑液（SF）生物标志物变得越来越重要。这项对照试验旨在评估接受全膝关节置换术（TKR）的重度OA患者与对照组（即年龄小于35岁、因急性半月板损伤接受膝关节镜检查的受试者）的SF蛋白质组之间的差异。
从接受全髋关节置换术的Kellgren Lawrence 3级和4级膝关节骨关节炎患者（研究组）以及半月板撕裂且无OA体征的接受关节镜手术的年轻患者（对照组）中收集滑膜样本。按照我们先前研究中定义的方案对样本进行处理和分析。所有患者均使用国际膝关节文献委员会（IKDC）主观膝关节评估（主要结局）、膝关节协会临床评分系统（KSS）、膝关节损伤和骨关节炎结局评分（KOOS）以及视觉模拟量表（VAS）进行疼痛临床评估。记录用药情况和合并症。所有患者均接受术前系列血液检查，包括全血细胞计数和C反应蛋白（CRP）。
滑膜样本分析显示，与对照样本相比，OA患者的纤维蛋白原β链（FBG）和α烯醇化酶1（ENO1）浓度存在显著差异。在骨关节炎患者中观察到临床评分、FBG和ENO1浓度之间存在显著相关性。
与非OA受试者相比，膝关节OA患者的滑液FBG和ENO1浓度存在显著差异。
