Nastulyavichus Alena, Tolordava Eteri, Kudryashov Sergey, Khmelnitskii Roman, Ionin Andrey
P. N. Lebedev Physics Institute of Russian Academy of Sciences, 119991 Moscow, Russia.
School of Natural Sciences and Mathematics, Ural Federal University, 620000 Ekaterinburg, Russia.
Materials (Basel). 2023 Jun 11;16(12):4309. doi: 10.3390/ma16124309.
In the present study, copper and silver nanoparticles with a concentration of 20 µg/cm were synthesized using the method of laser-induced forward transfer (LIFT). The antibacterial activity of the nanoparticles was tested against bacterial biofilms that are common in nature, formed by several types of microorganisms (mixed-species bacteria biofilms): , and The Cu nanoparticles showed complete inhibition of the bacteria biofilms used. In the course of the work, a high level of antibacterial activity was demonstrated by nanoparticles. This activity manifested in the complete suppression of the daily biofilm, with the number of bacteria decreasing by 5-8 orders of magnitude from the initial concentration. To confirm antibacterial activity, and determine reductions in cell viability, the Live/Dead Bacterial Viability Kit was used. FTIR spectroscopy revealed that after Cu NP treatment, there was in a slight shift in the region, which corresponded to fatty acids, indicating a decrease in the relative motional freedom of molecules.
在本研究中，采用激光诱导正向转移（LIFT）法合成了浓度为20µg/cm的铜和银纳米颗粒。测试了这些纳米颗粒对自然界常见的由几种微生物形成的细菌生物膜（混合菌种细菌生物膜）的抗菌活性： ，以及 铜纳米颗粒对所用细菌生物膜表现出完全抑制作用。在研究过程中，纳米颗粒展现出高水平的抗菌活性。这种活性表现为对日常生物膜的完全抑制，细菌数量从初始浓度减少了5 - 8个数量级。为了确认抗菌活性并确定细胞活力的降低情况，使用了活/死细菌活力试剂盒。傅里叶变换红外光谱（FTIR）显示，铜纳米颗粒处理后，对应脂肪酸的区域有轻微位移，表明分子的相对运动自由度降低。
