Kravtsov Dmitry, Gryadunov Dmitry, Shaskolskiy Boris
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Microorganisms. 2023 Jun 10;11(6):1547. doi: 10.3390/microorganisms11061547.
The aim of this work was to study the genetic diversity of the gonococcal genetic island (GGI) responsible for the type IV secretion system (T4SS) and the association of a functionally active GGI with antimicrobial resistance. An analysis of the GGI in a sample of 14,763 genomes of isolates from the Pathogenwatch database collected in 1996-2019 from 68 countries was performed. A model of GGI's genetic diversity that divides the global gonococcal population into fifty-one GGI clusters and three GGI superclusters based on the allele type of the gene and substitutions of the and genes for and has been proposed, reflecting differences among isolates in the T4SS functionality. The NG-MAST and MLST typing schemes (with accuracies of 91% and 83%, respectively) allowed the determination of both the presence of a GGI and the GGI cluster and, correspondingly, the structure of the GGI and the ability to secrete DNA. A statistically significant difference in the proportion of isolates resistant to ciprofloxacin, cefixime, tetracycline, and penicillin was found when comparing populations with a functional and a non-functional GGI. The presence of a functional GGI did not affect the proportion of azithromycin-resistant isolates.
这项工作的目的是研究负责IV型分泌系统（T4SS）的淋球菌遗传岛（GGI）的遗传多样性，以及功能活跃的GGI与抗菌药物耐药性之间的关联。对1996年至2019年从68个国家收集的Pathogenwatch数据库中14763株分离株的基因组样本中的GGI进行了分析。提出了一种GGI遗传多样性模型，该模型根据基因的等位基因类型以及基因和基因对和的替换，将全球淋球菌群体分为51个GGI簇和3个GGI超级簇，反映了分离株在T4SS功能上的差异。NG-MAST和MLST分型方案（准确率分别为91%和83%）能够确定GGI的存在和GGI簇，相应地，还能确定GGI的结构和分泌DNA的能力。在比较具有功能性和非功能性GGI的群体时，发现对环丙沙星、头孢克肟、四环素和青霉素耐药的分离株比例存在统计学上的显著差异。功能性GGI的存在并不影响对阿奇霉素耐药的分离株比例。
