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2020 - 2021年俄罗斯收集的分离株的阿奇霉素敏感性测试及分子研究

Azithromycin Susceptibility Testing and Molecular Investigation of Isolates Collected in Russia, 2020-2021.

作者信息

Kandinov Ilya, Shaskolskiy Boris, Kravtsov Dmitry, Vinokurova Alexandra, Gorshkova Sofya, Kubanov Alexey, Solomka Victoria, Shagabieva Julia, Deryabin Dmitry, Dementieva Ekaterina, Gryadunov Dmitry

机构信息

Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.

State Research Center of Dermatovenerology and Cosmetology, Russian Ministry of Health, Moscow 107076, Russia.

出版信息

Antibiotics (Basel). 2023 Jan 13;12(1):170. doi: 10.3390/antibiotics12010170.

DOI:10.3390/antibiotics12010170
PMID:36671371
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9854565/
Abstract

The aim of this work was to study the resistance to macrolides (azithromycin) in the modern Russian population of with the analysis of genetic resistance determinants. Azithromycin is not used to treat gonococcal infection in Russia. However, among 162 isolates collected in 2020-2021, 22 isolates (13.6%) were phenotypically resistant to azithromycin. Mutations in 23S rRNA genes were found only in two isolates; and genes were absent. Azithromycin resistance was shown to be predominantly associated with mutations in the and genes of the MtrCDE efflux pump and their mosaic alleles which may have formed due to a horizontal transfer from . A total of 30 types of alleles and 10 types of alleles were identified including mosaic variants. Matching between the and alleles was revealed to indicate the cooperative molecular evolution of these genes. A link between the and alleles and NG-MAST types was found only for NG-MAST 228 and 807, typical of in Russia. The high level of resistance to azithromycin in Russia may be related to the spread of multiple transferable resistance to antimicrobials regardless of their use in the treatment of gonococcal infection.

摘要

这项工作的目的是通过分析基因抗性决定因素来研究现代俄罗斯人群对大环内酯类药物（阿奇霉素）的抗性。在俄罗斯，阿奇霉素不用于治疗淋球菌感染。然而，在2020 - 2021年收集的162株分离株中，有22株（13.6%）对阿奇霉素表现出表型抗性。仅在两株分离株中发现了23S rRNA基因的突变；且未发现 和 基因。结果表明，阿奇霉素抗性主要与MtrCDE外排泵的 和 基因及其可能因从 水平转移而形成的嵌合等位基因中的突变有关。共鉴定出30种 等位基因类型和10种 等位基因类型，包括嵌合变体。发现 和 等位基因之间的匹配表明这些基因的协同分子进化。仅在俄罗斯典型的NG - MAST 228和807中发现了 和 等位基因与NG - MAST类型之间的联系。俄罗斯对阿奇霉素的高抗性水平可能与多种可转移的抗菌药物抗性的传播有关，而无论其是否用于治疗淋球菌感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/6485774fc18c/antibiotics-12-00170-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/b35c530703af/antibiotics-12-00170-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/ef03d6471082/antibiotics-12-00170-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/3aec28944b39/antibiotics-12-00170-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/6485774fc18c/antibiotics-12-00170-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/b35c530703af/antibiotics-12-00170-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/ef03d6471082/antibiotics-12-00170-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/3aec28944b39/antibiotics-12-00170-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eec1/9854565/6485774fc18c/antibiotics-12-00170-g004.jpg

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Molecular Mechanisms of Drug Resistance and Epidemiology of Multidrug-Resistant Variants of .多药耐药变体的耐药机制和流行病学。
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西班牙马德里一家三级医院应用 Allplex NG-DR 检测试剂盒（Seegene）检测淋病奈瑟菌中与大环内酯类和氟喹诺酮类耐药相关的突变的流行情况。
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