中国东部37株淋病奈瑟菌分离株的分子特征及携带淋球菌遗传岛的状况
Molecular Characteristics and Gonococcal Genetic Island Carrying Status of Thirty-Seven Isolates in Eastern China.
作者信息
Zhang Dan, Hu Mingpeng, Chi Shengying, Chen Han, Lin Chunchan, Yu Fangyou, Zheng Zhou
机构信息
Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
Ruian Traditional Chinese Medicine Hospital, Wenzhou, People's Republic of China.
出版信息
Infect Drug Resist. 2022 Nov 8;15:6545-6553. doi: 10.2147/IDR.S385079. eCollection 2022.
PURPOSE
To investigate the carrying situation of genetic island (GGI), and to understand the existence of GGI of different multilocus sequence types (MLST), so as to provide evidence for epidemiology.
METHODS
From January 2018 to December 2020, a total of 37 clinical isolates of were collected. Resistance to tetracycline, β-lactam, and azithromycin were measured. Genes in GGI (, and ) were amplified via polymerase chain reaction (PCR). All clinical isolates were subjected to MLST.
RESULTS
The GGI of were widespread, and the positive detection rates of and were all 81.08% (30/37). In this study, and were always detected positive together. No significant difference in the positive rate of the GGI between the azithromycin-sensitive and the resistance groups or between the β-lactam positive and negative groups ( > 0.05) was found; however, there was a significant difference between the high-level tetracycline-resistant group and the non-high-level resistant group (P < 0.05), with the carrier rates being 60.00% and 94.45%, respectively. Among the 37 isolates studied, 12 distinct MLST were determined, while MLST ST8123 occurred most frequently, accounting for 18.91% (7/37), followed by ST1928, ST7367 and ST7822, all 13.51% (5/37).
CONCLUSION
typed as ST1928, ST1901, ST1588 and ST7822, the GGI were all positive. These four types are more likely to become highly virulent strains.
目的
研究基因岛（GGI）的携带情况，了解不同多位点序列类型（MLST）的GGI存在情况，为流行病学提供依据。
方法
2018年1月至2020年12月，共收集37株临床分离株。检测其对四环素、β-内酰胺类和阿奇霉素的耐药性。通过聚合酶链反应（PCR）扩增GGI中的基因（、和）。所有临床分离株均进行MLST。
结果
的GGI广泛存在，和的阳性检出率均为81.08%（30/37）。本研究中，和总是同时检测为阳性。阿奇霉素敏感组和耐药组或β-内酰胺阳性组和阴性组之间GGI的阳性率无显著差异（>0.05）；然而，高水平四环素耐药组和非高水平耐药组之间存在显著差异（P<0.05），携带率分别为60.00%和94.45%。在研究的37株分离株中，确定了12种不同的MLST，其中MLST ST8123出现频率最高，占18.91%（7/37），其次是ST1928、ST7367和ST7822，均为13.51%（5/37）。
结论
分型为ST1928、ST1901、ST1588和ST7822的GGI均为阳性。这四种类型更有可能成为高毒力菌株。
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