Department of Botany, Mirpur University of Science and Technology, Mirpur 10250, Pakistan.
Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Molecules. 2023 Jun 7;28(12):4607. doi: 10.3390/molecules28124607.
The challenges in the production of metabolites of medicinal potential from wild plants include low yields, slow growth rates, seasonal variations, genetic variability and regulatory as well as ethical constraints. Overcoming these challenges is of paramount significance and interdisciplinary approaches and innovative strategies are prevalently applied to optimize phytoconstituents' production, enhance yield, biomass, ensure sustainable consistency and scalability. In this study, we investigated the effects of elicitation with yeast extract and calcium oxide nanoparticles (CaONPs) on in vitro cultures of (Roxb. ex Fleming) Karsten. Specifically, we examined the effects of different concentrations of CaONPs in combination with different concentrations of yeast extract on various parameters related to callus growth, antioxidant activity, biomass and phytochemical contents. Our results showed that elicitation with yeast extract and CaONPs had significant effects on the growth and characteristics of callus cultures of . The treatments involving yeast extract and CaONPs were found to be the most effective in increasing the contents of total flavonoid contents (TFC), total phenolic contents (TPC), amarogentin and mangiferin. These treatments also led to an improvement in the contents of total anthocyanin and alpha tocopherols. Additionally, the DPPH scavenging activity was significantly increased in the treated samples. Furthermore, the treatments involving elicitation with yeast extract and CaONPs also led to significant improvements in callus growth and characteristics. These treatments promoted callus response from an average to an excellent level and improved the color and nature of the callus from yellow to yellow-brown and greenish and from fragile to compact, respectively. The best response was observed in treatments involving 0.20 g/L yeast extract and 90 ug/L CaONPs. Overall, our findings suggest that elicitation with yeast extract and CaONPs can be a useful strategy for promoting the growth, biomass, phytochemical contents and antioxidant activity of callus cultures of in comparison to wild plant herbal drug samples.
从野生植物中生产具有药用潜力的代谢物所面临的挑战包括产量低、生长缓慢、季节性变化、遗传可变性以及监管和伦理方面的限制。克服这些挑战至关重要，目前普遍采用跨学科方法和创新策略来优化植物成分的生产，提高产量、生物量，确保可持续的一致性和可扩展性。在这项研究中，我们研究了酵母提取物和氧化钙纳米颗粒（CaONPs）对（Roxb. ex Fleming）Karsten 植物的体外培养的诱导作用。具体来说，我们研究了不同浓度的 CaONPs 与不同浓度的酵母提取物组合对与愈伤组织生长、抗氧化活性、生物量和植物化学物质含量相关的各种参数的影响。我们的结果表明，酵母提取物和 CaONPs 的诱导作用对 的愈伤组织培养的生长和特征有显著影响。酵母提取物和 CaONPs 的处理被发现是增加总黄酮含量（TFC）、总酚含量（TPC）、amarogentin 和 mangiferin 含量的最有效方法。这些处理还导致总花青素和α生育酚含量的提高。此外，处理过的样品的 DPPH 清除活性显著增加。此外，酵母提取物和 CaONPs 的诱导处理也导致愈伤组织生长和特征的显著改善。这些处理将愈伤组织的反应从一般水平提高到优秀水平，并分别使愈伤组织的颜色和性质从黄色变为黄棕色和绿色，从脆弱变为紧密。在涉及 0.20 g/L 酵母提取物和 90ug/L CaONPs 的处理中观察到最佳反应。总的来说，与野生植物草药样本相比，我们的研究结果表明，酵母提取物和 CaONPs 的诱导作用可以成为促进 的愈伤组织生长、生物量、植物化学物质含量和抗氧化活性的有效策略。
