Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.
Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman.
Artif Cells Nanomed Biotechnol. 2021 Dec;49(1):626-634. doi: 10.1080/21691401.2021.1984935.
Nano-elicitation is one among the prioritised strategies considered globally for sustainable and uniform production of industrially important medicinal compounds. (Thai basil), a renowned medicinal species is a reservoir of commercially vital metabolites and proved for its health assuring effects in cancer, diabetes, microbial and cardiovascular diseases. However, its consumption and industrial demand raised intent to divert towards better alternates for ensuring sustainable production of medicinal compounds. Herein, we investigated the comparative potential of metal oxide [copper oxide (CuO) and manganese oxide (MnO)] nanoparticles to elicit the biosynthesis of bioactive metabolites and antioxidative capacity of callus cultures. Results showed that callus grown on MS media supplemented with 10 mg/L CuO-NPs resulted in the highest biomass accumulation (FW: 172.8 g/L, DW: 16.7 g/L), phenolic contents (TPC: 27.5 mg/g DW), and flavonoid contents (TFC: 9.1 mg/g DW) along with antioxidant activities (DPPH: 94%, ABTS: 881 μM TEAC, FRAP: 386 μM TEAC) compared with MnO-NPs and control. Likewise, the Superoxide dismutase (SOD: 1.28 nM/min/mg FW) and Peroxidase (POD: 0.48 nM/min/mg FW) activities were also recorded maximum in CuO-NPs elicited cultures than MnO-NPs and control. Moreover, the HPLC results showed that rosmarinic acid (11.4 mg/g DW), chicoric acid (16.6 mg/g DW), eugenol (0.21 mg/g DW) was found optimum in cultures at 10 mg/L CuO-NPs. Overall, it can be concluded that CuO nanoparticles can be effectively used as a elicitor for biosynthesis of metabolites in callus cultures of (Thai basil). The study is indeed a contribution to the field that will help decoding the mechanism of action of CuO NPs. However, further molecular investigations are needed to fully develop understanding about the metabolic potential of and scalling up this protocol for bulkup production of bioactive compounds.
纳米激发是全球优先考虑的策略之一，旨在实现工业上重要药用化合物的可持续和均匀生产。罗勒（泰国罗勒）是一种著名的药用物种，是商业上重要代谢物的储存库，并已被证明对癌症、糖尿病、微生物和心血管疾病具有健康保障作用。然而，由于其消费和工业需求的增加，人们有意转向更好的替代品，以确保药用化合物的可持续生产。在这里，我们研究了金属氧化物（氧化铜（CuO）和氧化锰（MnO））纳米粒子的比较潜力，以激发愈伤组织培养物中生物活性代谢物的生物合成和抗氧化能力。结果表明，在补充有 10mg/L CuO-NPs 的 MS 培养基上生长的愈伤组织导致生物量积累（FW：172.8g/L，DW：16.7g/L）、酚含量（TPC：27.5mg/g DW）和类黄酮含量（TFC：9.1mg/g DW）最高，以及抗氧化活性（DPPH：94%，ABTS：881μM TEAC，FRAP：386μM TEAC）与 MnO-NPs 和对照相比。同样，CuO-NPs 诱导培养物中的超氧化物歧化酶（SOD：1.28nM/min/mg FW）和过氧化物酶（POD：0.48nM/min/mg FW）活性也高于 MnO-NPs 和对照。此外，HPLC 结果表明，在 10mg/L CuO-NPs 下，迷迭香酸（11.4mg/g DW）、菊苣酸（16.6mg/g DW）、丁香酚（0.21mg/g DW）的含量最高。总的来说，结果表明，CuO 纳米粒子可以有效地用作愈伤组织培养物中代谢物生物合成的激发剂。该研究确实为该领域做出了贡献，有助于解码 CuO NPs 的作用机制。然而，需要进一步的分子研究来充分了解罗勒的代谢潜力，并扩大该方案以批量生产生物活性化合物。
