Tyczkowski J K, Hamilton P B
Poult Sci. 1986 Jun;65(6):1141-5. doi: 10.3382/ps.0651141.
A model for the study of pigmentation in young chickens is described in which a white corn-soy based diet supplemented with varying amounts of free lutein (0, 5, 10, 20, 40, and 80 micrograms/g diet) was fed from hatching until 3 weeks of age. The carotenoid content of tissues dissected from chicks of the various groups was measured by high pressure liquid chromatography. In intestinal contents, three forms of lutein were found, with lutein monoester greater than free lutein greater than lutein diester. In the serum, free lutein (96%) and lutein monoester (4%) were found. In the liver, free lutein (80%), monoester (20%), and traces of diester were found. In the integument (toe web), diester greater than monoester approximately equal to free alcohol were found. In each tissue, the concentrations were directly proportional to the dietary concentration of free lutein. The simplest explanation of the data appears to be that part of the free lutein in the diet is esterified during its passage down the intestinal tract and, regardless of its status when absorbed, it is transported in the body as the free alcohol. When it enters depot sites such as the integument, lutein is deposited mainly as esters, presumably as the result of local enzymatic activity.
本文描述了一种用于研究雏鸡色素沉着的模型，即从孵化到3周龄期间，给雏鸡饲喂以白玉米-大豆为基础的日粮，并添加不同量的游离叶黄素（0、5、10、20、40和80微克/克日粮）。通过高压液相色谱法测定从各组雏鸡解剖得到的组织中的类胡萝卜素含量。在肠道内容物中，发现了三种形式的叶黄素，其中叶黄素单酯大于游离叶黄素大于叶黄素二酯。在血清中，发现了游离叶黄素（96%）和叶黄素单酯（4%）。在肝脏中，发现了游离叶黄素（80%）、单酯（20%）和痕量的二酯。在体表（趾蹼）中，发现二酯大于单酯，游离醇含量大致相等。在每个组织中，浓度与日粮中游离叶黄素的浓度成正比。对这些数据最简单的解释似乎是，日粮中的部分游离叶黄素在通过肠道时被酯化，并且无论其吸收时的状态如何，它在体内都以游离醇的形式运输。当它进入诸如体表等储存部位时，叶黄素主要以酯的形式沉积，推测这是局部酶活性的结果。
