Tyczkowski J K, Hamilton P B
Poult Sci. 1986 Aug;65(8):1526-31. doi: 10.3382/ps.0651526.
Young broiler chickens were fed from hatching until 3 weeks of age with a white corn-soy diet amended with varying amounts of lutein diester to supply 0, 5, 10, 20, 40, and 80 micrograms free lutein/g diet. The lutein diester was added as a stabilized, microencapsulated extract of marigold (Tagetes erecta) petals. The concentrations of lutein diester, lutein monoester, and lutein in the contents of the jejunum and large intestine and in serum, liver, and toe web from these birds were measured by high pressure liquid chromatography. The contents of the jejunum and large intestine contained a mixture of lutein diester, lutein monoester, and lutein. The serum contained lutein (approximately 90%), lutein monoester (approximately 10%), and traces of lutein diester. The liver contained the three carotenoid classes in ratios reflecting the serum ratios. The ratios in toe web, an integumentary depot site, were reversed with lutein diester much greater than lutein monoester greater than lutein. The concentrations of each class in each tissue bore a linear relationship to the concentration of lutein diester in the diet. A simple explanation for these data is that the dietary lutein diester was hydrolyzed mainly to lutein, which was absorbed through the intestinal wall into the blood stream where it was transported to the liver, a storage site, and to the integumentary sites where it is esterified to lutein diester which is the main depot form.
从孵化开始至3周龄，给幼龄肉鸡饲喂添加了不同量叶黄素二酯的白玉米 - 大豆日粮，以使日粮中游离叶黄素的含量分别为0、5、10、20、40和80微克/克。叶黄素二酯以万寿菊（孔雀草）花瓣的稳定微囊化提取物形式添加。通过高压液相色谱法测定这些鸡的空肠和大肠内容物以及血清、肝脏和趾蹼中叶黄素二酯、叶黄素单酯和叶黄素的浓度。空肠和大肠内容物含有叶黄素二酯、叶黄素单酯和叶黄素的混合物。血清中含有叶黄素（约90%）、叶黄素单酯（约10%）和痕量叶黄素二酯。肝脏中三种类胡萝卜素的比例反映了血清中的比例。在作为体表储存部位的趾蹼中，比例则相反，叶黄素二酯远大于叶黄素单酯大于叶黄素。每个组织中各类胡萝卜素的浓度与日粮中叶黄素二酯的浓度呈线性关系。对这些数据的一个简单解释是，日粮中的叶黄素二酯主要水解为叶黄素，叶黄素通过肠壁吸收进入血流，然后被转运到作为储存部位的肝脏以及体表部位，在体表部位叶黄素被酯化为叶黄素二酯，这是主要的储存形式。
