Department of Bioanalytics, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090 Lublin, Poland.
Department of Meat Technology, Poznan University of Life Sciences, ul. Wojska Polskiego 31, 60-624 Poznan, Poland.
Molecules. 2023 Jun 14;28(12):4754. doi: 10.3390/molecules28124754.
The authenticity of food products marketed as health-promoting foods-especially unrefined, cold-pressed seed oils-should be controlled to ensure their quality and safeguard consumers and patients. Metabolomic profiling using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-QTOF) was employed to identify authenticity markers for five types of unrefined, cold-pressed seed oils: black seed oil ( L.), pumpkin seed oil ( L.), evening primrose oil ( L.), hemp oil ( L.) and milk thistle oil (). Of the 36 oil-specific markers detected, 10 were established for black seed oil, 8 for evening primrose seed oil, 7 for hemp seed oil, 4 for milk thistle seed oil and 7 for pumpkin seed oil. In addition, the influence of matrix variability on the oil-specific metabolic markers was examined by studying binary oil mixtures containing varying volume percentages of each tested oil and each of three potential adulterants: sunflower, rapeseed and sesame oil. The presence of oil-specific markers was confirmed in 7 commercial oil mix products. The identified 36 oil-specific metabolic markers proved useful for confirming the authenticity of the five target seed oils. The ability to detect adulterations of these oils with sunflower, rapeseed and sesame oil was demonstrated.
应控制作为保健品销售的食品产品（尤其是未精炼、冷榨的种子油）的真实性，以确保其质量并保护消费者和患者。采用液相色谱-四极杆飞行时间质谱联用（LC-QTOF）代谢组学分析方法，鉴定了 5 种未精炼、冷榨的种子油（黑籽油、南瓜籽油、月见草油、大麻籽油和奶蓟籽油）的真实性标志物。在所检测到的 36 种油特异性标志物中，有 10 种被确定为黑籽油标志物，8 种为月见草籽油标志物，7 种为大麻籽油标志物，4 种为奶蓟籽油标志物，7 种为南瓜籽油标志物。此外，通过研究含有每种测试油和 3 种潜在掺杂物（葵花籽油、菜籽油和芝麻油）不同体积百分比的二元油混合物，考察了基质变异性对油特异性代谢标志物的影响。在 7 种商业油混合产品中证实了存在油特异性标志物。鉴定出的 36 种油特异性代谢标志物可用于确认这 5 种目标种子油的真实性。证明了这些油与葵花籽油、菜籽油和芝麻油掺假的检测能力。
