用不同溶剂进行分级后对的多酚进行表征及其体外生物活性研究。

Characterization of Polyphenols from after Fractionation with Different Solvents and Study of Their In Vitro Biological Activity.

机构信息

Department of Organic Chemistry, Faculty of Chemistry, University of Plovdiv, 4000 Plovdiv, Bulgaria.

出版信息

Molecules. 2023 Jun 16;28(12):4816. doi: 10.3390/molecules28124816.

DOI:10.3390/molecules28124816

PMID:37375371

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10300873/

Abstract

In the present work, we have investigated the polyphenolic composition of from Bulgaria. The polyphenols were fractionated with solvents of varying polarity (-hexane, chloroform, ethyl acetate, and -butanol). The fractions were analyzed by HPLC-PDA and UHPLC-MS. The ethyl acetate fraction contained mono- and di-glycosides of quercetin, di-glycosides of kaempferol, and isorhamnetin and monoglycosides of hispidulin and jaceosidine. We found quercetin triglycosides in the butanol fraction. The ethyl acetate and butanol fractions contained 168.82 mg/g Extr and 67.21 mg/g Extr of quercetin glycosides, respectively. The main components of the polyphenolic complex in were 6-methoxyflavones (355.47 mg/g Extr), which were found in the chloroform fraction. The flavonoids pectolinarigenin, demethylnobiletin, and isosinensetin, and the glycosides of quercetin (triglycosides, acylglycosides), kaempferol, isorhamnetin, hispidiulin, and jaceosidine, were discovered and reported in for the first time. We used in vitro methods to assess the biological activity against oxidative stress (hydrogen peroxide scavenging activity (HPSA) and hydroxyl radical scavenging activity (HRSA)), nitrosative stress (nitric oxide scavenging activity (NOSA)), anti-inflammatory activity (IAD inhibition), and anti-tryptic activity (ATA). Quercetin mono- and di-glycosides exhibited greater HPSA and HRSA (IC = 39.18, 105.03 µg/mL), while 6-methoxyflavones had a greater NOSA (IC = 146.59 µg/mL). The same components showed the highest ATA (IC ranging from 116.23 to 202.44 µg/mL).

摘要

在本工作中，我们研究了保加利亚的多酚组成。多酚用不同极性的溶剂（正己烷、氯仿、乙酸乙酯和正丁醇）进行分级。各馏分用 HPLC-PDA 和 UHPLC-MS 进行分析。乙酸乙酯馏分含有槲皮素的单糖苷和二糖苷、山奈酚的二糖苷和异鼠李素及次芹素的单糖苷。我们在正丁醇馏分中发现了槲皮素三糖苷。乙酸乙酯和正丁醇馏分分别含有 168.82 和 67.21 mg/g 提取物的槲皮素糖苷。的多酚复合物的主要成分是 6-甲氧基黄酮（355.47 mg/g 提取物），在氯仿馏分中发现了该成分。还首次在中发现并报道了黄酮类化合物 pectolinarigenin、去甲川陈皮素和异补骨脂素，以及槲皮素（三糖苷、酰基糖苷）、山奈酚、异鼠李素、次芹素和筋骨草苷的糖苷。我们使用体外方法评估了其对氧化应激（过氧化氢清除活性（HPSA）和羟基自由基清除活性（HRSA））、硝化应激（一氧化氮清除活性（NOSA））、抗炎活性（IAD 抑制）和抗胰蛋白酶活性（ATA）的生物活性。槲皮素单糖苷和二糖苷表现出更强的 HPSA 和 HRSA（IC = 39.18、105.03 µg/mL），而 6-甲氧基黄酮具有更强的 NOSA（IC = 146.59 µg/mL）。相同的成分表现出最高的 ATA（IC 范围为 116.23 至 202.44 µg/mL）。