Zhang Wenting, Liang Guixing, Cheng Zhenyu, Guo Yunqing, Jiang Boda, Liu Tingjiang, Liao Weidong, Lu Qin, Wen Guoyuan, Zhang Tengfei, Luo Qingping
Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 4R2, Canada.
Pathogens. 2023 May 31;12(6):790. doi: 10.3390/pathogens12060790.
Pullorum disease, caused by Pullorum (. Pullorum), is one of the most serious infectious diseases in the poultry industry. is traditionally used in Eastern Asian countries to treat various intestinal diseases. However, the anti-infection mechanism of is not very clear. In this study, we evaluated the anti-infective effects on Pullorum of aqueous extract (FPAE) in chickens. FPAE significantly reduced Pullorum growth in vitro. At the cellular level, FPAE reduced Pullorum adhesion and invasion on DF-1 cells but did not affect its intracellular survival or replication in macrophages. Further investigation revealed that FPAE inhibited the transcription of T3SS-1 genes, which is the main virulence factor that mediates Pullorum adhesion and invasion in host cells. The results suggest that the anti-infective effect of FPAE likely occurs through the inhibition of Pullorum T3SS-1, thereby impairing its ability to adhere to and invade cells. Further, we evaluated its therapeutic effect on animal models (Jianghan domestic chickens) and found that FPAE reduced the bacterial loads in organs and decreased the mortality and weight loss of infected chickens. Our findings provide novel insights into the potential development of FPAE against Pullorum as an effective anti-virulence therapeutic substitute for antibiotics.
鸡白痢是由鸡白痢沙门氏菌（Salmonella Pullorum）引起的，是家禽业中最严重的传染病之一。在东亚国家，传统上使用[某种物质]来治疗各种肠道疾病。然而，[某种物质]的抗感染机制尚不清楚。在本研究中，我们评估了[某种物质]水提取物（FPAE）对鸡白痢沙门氏菌的抗感染作用。FPAE在体外显著抑制了鸡白痢沙门氏菌的生长。在细胞水平上，FPAE减少了鸡白痢沙门氏菌对DF-1细胞的粘附和侵袭，但不影响其在巨噬细胞内的存活或复制。进一步研究表明，FPAE抑制了T3SS-1基因的转录，T3SS-1基因是介导鸡白痢沙门氏菌在宿主细胞中粘附和侵袭的主要毒力因子。结果表明，FPAE的抗感染作用可能是通过抑制鸡白痢沙门氏菌的T3SS-1，从而损害其粘附和侵袭细胞的能力。此外，我们评估了其对动物模型（江汉土鸡）的治疗效果，发现FPAE降低了器官中的细菌载量，降低了感染鸡的死亡率和体重减轻。我们的研究结果为FPAE作为抗生素的有效抗毒力治疗替代品对抗鸡白痢沙门氏菌的潜在开发提供了新的见解。
