Caspe Sergio Gaston, Ewing David Andrew, Livingstone Morag, Underwood Clare, Milne Elspeth, Sargison Neil Donald, Wattegedera Sean Ranjan, Longbottom David
Moredun Research Institute, Penicuik EH26 0PZ, UK.
Estación Experimental Mercedes, Instituto Nacional de Tecnología Agropecuaria (INTA), Corrientes W3400, Argentina.
Pathogens. 2023 Jun 19;12(6):846. doi: 10.3390/pathogens12060846.
The enzootic abortion of ewes, caused by the bacterium (), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with . Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in -infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing.
由细菌（）引起的母羊地方性流产是绵羊流产的主要原因之一。有多种促成因素，包括衣原体生长、宿主免疫反应和激素平衡，这些因素导致不同的妊娠结局，如流产、出生后可能死亡的弱羔羊或健康羔羊。本研究旨在确定在实验感染（）时，双胎绵羊（两只羔羊均死亡；一死一活；两只均存活）中免疫细胞浸润的表型模式与不同妊娠结局之间的关系。分娩后收集绵羊的子宫和胎盘。通过免疫组织化学和原位杂交对所有样本进行特定免疫细胞特征分析，包括细胞表面抗原、调节性T（Treg）细胞相关转录因子和细胞因子。其中一些免疫抗原首次在绵羊生殖组织中进行评估。辅助性T细胞/Treg细胞的差异模式在胎盘中显示出显著的组间效应。这表明淋巴细胞亚群平衡在影响感染（）的绵羊不同妊娠结局中可能发挥的潜在作用。本研究提供了关于早产流产或产羔时绵羊母胎界面免疫反应的新颖详细信息。
