Department of Microbiology, College of Veterinary Medicine, University of Sulaimani , New Sulaimani, Sulaymaniyah, Kurdistan Region , Northern Iraq.
Charmo University , Sulaimani , Kurdistan Region-Iraq.
Pol J Microbiol. 2020;69(1):1-7. doi: 10.33073/pjm-2020-009.
Abortion in small ruminants is a significant problem in Iraq and causes severe economic losses in sheep farms. causes enzootic abortion in ewes and is associated with reproductive problems in sheep in Sulaimani province - Northern Iraq. During a lambing season in 2017, abortion was widespread among several sheep flocks in different regions of Sulaimani (Kalar, Said Sadiq, and Chamchamal), and was one of the causes. Accordingly, we carried out this study to isolate and identify in aborted ewes in these regions. We collected 30 samples of aborted fetuses from five herds in which abortions had been observed. The pathogen isolation was done by inoculation into embryonated chicken eggs and conventional PCR was used to identify in clinical specimens. was identified in one of the 30 aborted fetuses (3.33%) from the Kalar district, and all the remaining 29 samples (96.66%) were found positive to . The gene encoding the outer membrane protein of was sequenced and got the accession number MK643153 in NCBI GenBank. The sequence was named strain Sul/2017. Our isolate showed 99.79% homology with Sul/014 (accession No. KY399850) and differed from the latter by two amino acid substitutions at E115K and K259N. The topology of the phylogenetic tree based on the gene showed that the isolate belongs to and has a common ancestor with isolates of sheep in Iraq and Tunisia with accession numbers KY399850 and HQ62243, respectively. Abortion in small ruminants is a significant problem in Iraq and causes severe economic losses in sheep farms. causes enzootic abortion in ewes and is associated with reproductive problems in sheep in Sulaimani province – Northern Iraq. During a lambing season in 2017, abortion was widespread among several sheep flocks in different regions of Sulaimani (Kalar, Said Sadiq, and Chamchamal), and was one of the causes. Accordingly, we carried out this study to isolate and identify in aborted ewes in these regions. We collected 30 samples of aborted fetuses from five herds in which abortions had been observed. The pathogen isolation was done by inoculation into embryonated chicken eggs and conventional PCR was used to identify in clinical specimens. was identified in one of the 30 aborted fetuses (3.33%) from the Kalar district, and all the remaining 29 samples (96.66%) were found positive to . The gene encoding the outer membrane protein of was sequenced and got the accession number MK643153 in NCBI GenBank. The sequence was named strain Sul/2017. Our isolate showed 99.79% homology with Sul/014 (accession No. KY399850) and differed from the latter by two amino acid substitutions at E115K and K259N. The topology of the phylogenetic tree based on the gene showed that the isolate belongs to and has a common ancestor with isolates of sheep in Iraq and Tunisia with accession numbers KY399850 and HQ62243, respectively.
在伊拉克，小反刍动物流产是一个严重的问题，给绵羊养殖场造成了严重的经济损失。 导致绵羊地方性流产，并与伊拉克苏莱曼尼亚省的绵羊繁殖问题有关。在 2017 年的一个产羔季节，不同地区（卡拉勒、赛义德·萨迪克和查姆查马尔）的几个绵羊群中广泛发生流产， 是其中一个原因。因此，我们进行了这项研究，以分离和鉴定这些地区流产母羊中的 。我们从观察到流产的五个羊群中收集了 30 个流产胎儿样本。通过接种鸡胚进行病原体分离，并用常规 PCR 鉴定临床标本中的 。在卡拉勒区的 30 个流产胎儿中，有 1 个（3.33%）鉴定为 ，其余 29 个（96.66%）均为阳性。 编码 的外膜蛋白的基因进行测序，并在 NCBI GenBank 中获得 MK643153 的登录号。该序列被命名为 Sul/2017 株。我们的分离株与 Sul/014（登录号 KY399850）显示出 99.79%的同源性，并且在 E115K 和 K259N 处有两个氨基酸取代与后者不同。基于 基因的系统发育树拓扑结构表明，该分离株属于 ，与伊拉克和突尼斯绵羊的分离株具有共同的祖先，其登录号分别为 KY399850 和 HQ62243。
