Department of Oriental Medicine Biotechnology, College of Life Sciences and Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Gyeonggi-do, Republic of Korea.
School of Pharmacy, Sungkyunkwan University, Suwon 16419, Gyeonggi-do, Republic of Korea.
Nutrients. 2023 Jun 9;15(12):2685. doi: 10.3390/nu15122685.
Diabetes is a prevalent and debilitating metabolic disorder affecting a large population worldwide. The condition is characterized by insulin resistance and impaired function of pancreatic β-cells, leading to elevated blood glucose levels. In this study, the antidiabetic effects of extract (EAE) on zebrafish with damaged pancreatic islets caused by insulin resistance were investigated. The study utilized the zebrafish model to monitor live pancreatic islets. RNA sequencing was also conducted to determine the mechanism by which EAE exerts its antidiabetic effect. The results showed that EAE was effective in recovering reduced islets in excess insulin-induced zebrafish. The effective concentration at 50% (EC) of EAE was determined to be 0.54 μg/mL, while the lethal concentration at 50% (LC) was calculated as 202.5 μg/mL. RNA sequencing indicated that the mode of action of EAE is related to its ability to induce mitochondrial damage and suppress endoplasmic reticulum stress. The findings of this study demonstrate the efficacy and therapeutic potential of EAE in treating insulin resistance in zebrafish. The results suggest that EAE may offer a promising approach for the management of diabetes by reducing mitochondrial damage and suppressing endoplasmic reticulum stress. Further research is required to establish the clinical application of EAE in diabetic patients.
糖尿病是一种普遍存在且衰弱性的代谢紊乱疾病，影响着全球大量人口。该疾病的特征是胰岛素抵抗和胰腺β细胞功能受损，导致血糖水平升高。在这项研究中，研究了 提取物（EAE）对胰岛素抵抗引起的受损胰腺胰岛的斑马鱼的抗糖尿病作用。该研究利用斑马鱼模型来监测活胰腺胰岛。还进行了 RNA 测序，以确定 EAE 发挥抗糖尿病作用的机制。结果表明，EAE 可有效恢复过量胰岛素诱导的斑马鱼中减少的胰岛。EAE 的有效浓度为 50%（EC）为 0.54 μg/mL，而 50%的致死浓度（LC）计算为 202.5 μg/mL。RNA 测序表明，EAE 的作用模式与其诱导线粒体损伤和抑制内质网应激的能力有关。这项研究的结果证明了 EAE 在治疗斑马鱼胰岛素抵抗方面的疗效和治疗潜力。研究结果表明，EAE 可能通过减少线粒体损伤和抑制内质网应激为糖尿病患者的管理提供一种有前途的方法。需要进一步的研究来确定 EAE 在糖尿病患者中的临床应用。
