Hemmingsen Lisa Myrseth, Panzacchi Virginia, Kangu Lloyd Mbugua, Giordani Barbara, Luppi Barbara, Škalko-Basnet Nataša
Department of Pharmacy, University of Tromsø-The Arctic University of Norway, Universitetsvegen 57, 9037 Tromsø, Norway.
Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Pharmaceuticals (Basel). 2023 May 25;16(6):790. doi: 10.3390/ph16060790.
The main focus when considering treatment of non-healing and infected wounds is tied to the microbial, particularly bacterial, burden within the wound bed. However, as fungal contributions in these microbial communities become more recognized, the focus needs to be broadened, and the remaining participants in the complex wound microbiome need to be addressed in the development of new treatment strategies. In this study, lecithin/chitosan nanoparticles loaded with clotrimazole were tailored to eradicate one of the most abundant fungi in the wound environment, namely . Moreover, this investigation was extended to the building blocks and their organization within the delivery system. In the evaluation of the novel nanoparticles, their compatibility with keratinocytes was confirmed. Furthermore, these biocompatible, biodegradable, and non-toxic carriers comprising clotrimazole (~189 nm, 24 mV) were evaluated for their antifungal activity through both disk diffusion and microdilution methods. It was found that the activity of clotrimazole was fully preserved upon its incorporation into this smart delivery system. These results indicate both that the novel carriers for clotrimazole could serve as a therapeutic alternative in the treatment of fungi-infected wounds and that the building blocks and their organization affect the performance of nanoparticles.
在考虑治疗难愈合及感染伤口时，主要关注点与伤口床内的微生物负担相关，尤其是细菌负担。然而，随着真菌在这些微生物群落中的作用得到更多认可，关注点需要拓宽，在制定新的治疗策略时需要考虑复杂伤口微生物群中的其他成员。在本研究中，负载克霉唑的卵磷脂/壳聚糖纳米颗粒旨在根除伤口环境中最常见的真菌之一，即 。此外，该研究还扩展到了递送系统中的组成成分及其组织方式。在对新型纳米颗粒的评估中，证实了它们与角质形成细胞的相容性。此外，通过纸片扩散法和微量稀释法评估了这些包含克霉唑（约189纳米，24毫伏）的生物相容性、可生物降解且无毒的载体的抗真菌活性。结果发现，克霉唑掺入这种智能递送系统后其活性得以完全保留。这些结果表明，新型克霉唑载体可作为治疗真菌感染伤口的一种治疗选择，且组成成分及其组织方式会影响纳米颗粒的性能。
