Miller School of Medicine, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery Wound Healing Research Laboratory Miami, University of Miami, Miami, 33136, FL, United States.
BMC Microbiol. 2022 Feb 4;22(1):45. doi: 10.1186/s12866-022-02460-x.
Candida albicans is a common cause of opportunistic mycoses worldwide and a major contributor in wound infections. The purpose of this study was to establish a fungal wound model and analyze the effects of a common antifungal agent against the proliferation of three C. albicans strains. Second degree burns were created, and then inoculated with one of three different C. albicans ATCC strains: 10261 reference strain, 64550 fluconazole resistant and 26310 fluconazole sensitive. After fungal inoculation, every wound was covered with dressings for 4 h to allow fungal colonization on every wound bed. After 4 h, the dressings were removed, and each wound was treated either once or twice daily with a topical terbinafine hydrochloride or left untreated. On days 2, 4 and 7 post inoculation, three wounds from each treatment group were scrub cultured and quantified. On day 2, wounds infected with the sensitive strains 26310 and 10261 and treated twice showed a significant reduction when compared against those infected wounds receiving once daily treatment. On day 4, wounds which were infected with C. albicans fluconazole sensitive (ATCC 26310) showed a significant reduction in fungal cell counts with treatment applied twice daily. A significant reduction in the colony counts was exhibited in all three strains at the seventh day with active as compared to the non-treated wounds. Twice daily treatment resulted in a lower fungal count than once daily treatment. Neither treatment was able to entirely eradicate C. albicans during the duration of this study. Establishing a reliable fungal wound model will help in the translational goal of identifying new antifungal that could be used clinically by wound care providers.
白色念珠菌是一种常见的机会性真菌，在世界各地引起真菌感染，也是伤口感染的主要原因。本研究旨在建立一种真菌性伤口模型，并分析一种常用抗真菌药物对三种白色念珠菌菌株增殖的影响。制造二度烧伤模型，然后接种三种不同的白色念珠菌 ATCC 菌株之一：10261 参考菌株、64550 氟康唑耐药株和 26310 氟康唑敏感株。真菌接种后，每个伤口用敷料覆盖 4 小时，以使真菌定植于每个伤口床。4 小时后，去除敷料，每天用特比萘芬盐酸盐局部涂抹一次或两次，或者不处理。在接种后第 2、4 和 7 天，从每个治疗组中取出三个伤口进行刷洗培养和定量分析。在第 2 天，与接受每日一次治疗的感染伤口相比，用特比萘芬盐酸盐处理两次的敏感菌株 26310 和 10261 感染的伤口显示出明显减少。在第 4 天，用氟康唑敏感（ATCC 26310）感染的伤口每天两次处理显示出真菌细胞计数的显著减少。与未处理的伤口相比，在第 7 天，所有三种菌株的菌落计数都显著减少，活性治疗组与非治疗组相比，真菌计数明显减少。与每日一次处理相比，每日两次处理导致真菌计数降低。在本研究期间，两种处理方法均未能完全消除白色念珠菌。建立可靠的真菌性伤口模型将有助于鉴定新的抗真菌药物，以便为伤口护理人员提供临床应用。
