Abou-Shanab Ahmed M, Fytory Mostafa, Shouman Shaimaa, Atta Dina, Khairy Asmaa, Salah Radwa Ayman, Idris Omaima, El-Badri Nagwa
Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology Giza 12578 Egypt
Biomedical Sciences Program, University of Science and Technology, Zewail City of Science and Technology Giza 12578 Egypt.
Nanoscale Adv. 2025 Jun 26. doi: 10.1039/d5na00222b.
The human amniotic membrane (hAM) is a promising therapeutic source in regenerative medicine due to its rich content of growth factors and bioactive molecules. Its clinical application, however, has remained limited by challenges in controlled delivery and compositional complexity. To address these issues, we developed a nanoparticle-based formulation (NF-hAM) to encapsulate hAM proteins and enhance their targeted delivery to mesenchymal stem cells (MSCs) and fibroblasts. NF-hAM was synthesized using soy lecithin and chitosan, forming spherical nanoparticles with well-defined morphology. Physicochemical characterization confirmed successful protein encapsulation and nanoparticle stability. studies demonstrated that NF-hAM was biocompatible and significantly promoted the proliferation of bone marrow-derived MSCs (BMSCs), adipose-derived MSCs (AMSCs), and human skin fibroblasts (HSFs). In HSFs, NF-hAM also induced cytoskeletal remodeling, suggesting enhanced cellular activity and functional responsiveness. Additionally, NF-hAM enhanced the osteogenic and adipogenic differentiation potential of BMSCs. Metabolic analyses further revealed that NF-hAM stimulated glycolytic activity, a key metabolic pathway associated with self-renewal and proliferation. Our findings highlight the potential of NF-hAM as a therapeutic nanomedicine for regenerative medical applications.
人羊膜（hAM）因其富含生长因子和生物活性分子，是再生医学中一种很有前景的治疗来源。然而，其临床应用一直受到可控递送和成分复杂性方面挑战的限制。为了解决这些问题，我们开发了一种基于纳米颗粒的制剂（NF-hAM），用于封装hAM蛋白并增强其向间充质干细胞（MSCs）和成纤维细胞的靶向递送。NF-hAM是使用大豆卵磷脂和壳聚糖合成的，形成了形态明确的球形纳米颗粒。物理化学表征证实了蛋白的成功封装和纳米颗粒的稳定性。研究表明，NF-hAM具有生物相容性，并显著促进了骨髓间充质干细胞（BMSCs）、脂肪间充质干细胞（AMSCs）和人皮肤成纤维细胞（HSFs）的增殖。在HSFs中，NF-hAM还诱导了细胞骨架重塑，表明细胞活性和功能反应性增强。此外，NF-hAM增强了BMSCs的成骨和成脂分化潜能。代谢分析进一步表明，NF-hAM刺激了糖酵解活性，这是一种与自我更新和增殖相关的关键代谢途径。我们的研究结果突出了NF-hAM作为一种用于再生医学应用的治疗性纳米药物的潜力。
