Virág Eszter, Kiniczky Márta, Kutasy Barbara, Nagy Ágnes, Pallos József Péter, Laczkó Levente, Freytag Csongor, Hegedűs Géza
Research Institute for Medicinal Plants and Herbs Ltd., Lupaszigeti Str 4, 2011 Budakalász, Hungary.
EduCoMat Ltd., Iskola Str 12A, 8360 Keszthely, Hungary.
Plants (Basel). 2023 Jun 14;12(12):2308. doi: 10.3390/plants12122308.
Plant immunological memory, priming, is a defense mechanism that can be triggered by external stimuli, leading to the activation of biochemical pathways and preparing plants for disease resistance. Plant conditioners improve yield and crop quality through nutrient efficiency and abiotic stress tolerance, which is enhanced by the addition of resistance- and priming-induced compounds. Based on this hypothesis, this study aimed to investigate plant responses to priming actives of different natures, including salicylic acid and beta-aminobutyric acid, in combination with the plant conditioning agent ELICE Vakcina. Phytotron experiments and RNA-Seq analyses of differentially expressed genes using the combinations of these three investigated compounds were performed in a barley culture to investigate possible synergistic relationships in the genetic regulatory network. The results indicated a strong regulation of defense responses, which was enhanced by supplemental treatments; however, both synergistic and antagonistic effects were enhanced with one or two components, depending on the supplementation. The overexpressed transcripts were functionally annotated to assess their involvement in jasmonic acid and salicylic acid signaling; however, their determinant genes were highly dependent on the supplemental treatments. Although the effects overlapped, the potential effects of -priming the two supplements tested could be largely separated.
植物免疫记忆，即引发作用，是一种防御机制，可由外部刺激触发，导致生化途径的激活，并使植物为抗病性做好准备。植物调理剂通过养分效率和非生物胁迫耐受性提高产量和作物品质，添加抗性和引发诱导化合物可增强这种耐受性。基于这一假设，本研究旨在探究植物对不同性质引发活性物质（包括水杨酸和β-氨基丁酸）与植物调理剂ELICE Vakcina组合的反应。在大麦培养中进行了植物生长箱实验，并使用这三种研究化合物的组合对差异表达基因进行RNA测序分析，以研究遗传调控网络中可能的协同关系。结果表明防御反应受到强烈调控，补充处理可增强这种调控；然而，根据补充情况，一种或两种成分会增强协同效应和拮抗效应。对过表达转录本进行功能注释，以评估它们在茉莉酸和水杨酸信号传导中的作用；然而，它们的决定基因高度依赖于补充处理。尽管效果重叠，但所测试的两种补充剂引发的潜在效果在很大程度上可以区分。
