Kuznetsova Yulia L, Gushchina Ksenya S, Lobanova Karina S, Chasova Victoria O, Egorikhina Marfa N, Grigoreva Alexandra O, Malysheva Yulia B, Kuzmina Daria A, Farafontova Ekaterina A, Linkova Daria D, Rubtsova Yulia P, Semenycheva Luydmila L
Faculty of Chemistry, National Research Lobachevsky State University of Nizhny Novgorod, 23, Gagarin Ave., 603022 Nizhny Novgorod, Russia.
Federal State Budgetary Educational Institution of Higher Education, Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia.
Polymers (Basel). 2023 Jun 8;15(12):2618. doi: 10.3390/polym15122618.
Polymerization of methyl methacrylate (MMA) in aqueous collagen (Col) dispersion was studied in the presence of tributylborane (TBB) and -quinone: 2,5-di-tert-butyl--benzoquinone (2,5-DTBQ), -benzoquinone (BQ), duroquinone (DQ), and -naphthoquinone (NQ). It was found that this system leads to the formation of a grafted cross-linked copolymer. The inhibitory effect of -quinone determines the amount of unreacted monomer, homopolymer, and percentage of grafted poly(methyl methacrylate) (PMMA). The synthesis combines two approaches to form a grafted copolymer with a cross-linked structure-"grafting to" and "grafting from". The resulting products exhibit biodegradation under the action of enzymes, do not have toxicity, and demonstrate a stimulating effect on cell growth. At the same time, the denaturation of collagen occurring at elevated temperatures does not impair the characteristics of copolymers. These results allow us to present the research as a scaffold chemical model. Comparison of the properties of the obtained copolymers helps to determine the optimal method for the synthesis of scaffold precursors-synthesis of a collagen and poly(methyl methacrylate) copolymer at 60 °C in a 1% acetic acid dispersion of fish collagen with a mass ratio of the components collagen:MMA:TBB:2,5-DTBQ equal to 1:1:0.015:0.25.
在三丁基硼（TBB）和醌类物质（2,5-二叔丁基-对苯醌（2,5-DTBQ）、对苯醌（BQ）、杜醌（DQ）和萘醌（NQ））存在的情况下，研究了甲基丙烯酸甲酯（MMA）在水性胶原蛋白（Col）分散体中的聚合反应。发现该体系会导致接枝交联共聚物的形成。醌类物质的抑制作用决定了未反应单体、均聚物的量以及接枝聚甲基丙烯酸甲酯（PMMA）的百分比。该合成结合了两种形成具有交联结构的接枝共聚物的方法——“接枝到”和“接枝自”。所得产物在酶的作用下表现出生物降解性，无毒，并对细胞生长具有刺激作用。同时，在高温下发生的胶原蛋白变性不会损害共聚物的特性。这些结果使我们能够将该研究作为一种支架化学模型呈现。对所得共聚物性能的比较有助于确定合成支架前体的最佳方法——在60℃下，在鱼胶原蛋白的1%乙酸分散体中合成胶原蛋白与聚甲基丙烯酸甲酯的共聚物，其中胶原蛋白、MMA、TBB、2,5-DTBQ的质量比为1:1:0.015:0.25。
