Kuznetsova Yulia L, Sustaeva Karina S, Mitin Alexander V, Zakharychev Evgeniy A, Egorikhina Marfa N, Chasova Victoria O, Farafontova Ekaterina A, Kobyakova Irina I, Semenycheva Lyudmila L
Federal State Budgetary Educational Institution of Higher Education, Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia.
Department of Organic Chemistry, Faculty of Chemistry, National Research Lobachevsky State University of Nizhny Novgorod, 23, Gagarin Ave., 603022 Nizhny Novgorod, Russia.
Polymers (Basel). 2022 Nov 13;14(22):4900. doi: 10.3390/polym14224900.
Graft copolymers of collagen and polyacrylamide (PAA) were synthesized in a suspension of acetic acid dispersion of fish collagen and acrylamide (AA) in the presence of tributylborane (TBB). The characteristics of the copolymers were determined using infrared spectroscopy and gel permeation chromatography (GPC). Differences in synthesis temperature between 25 and 60 °C had no significant effect on either proportion of graft polyacrylamide generated or its molecular weight. However, photomicrographs taken with the aid of a scanning electron microscope showed a breakdown of the fibrillar structure of the collagen within the copolymer at synthesis temperatures greater than 25 °C. The mechanical properties of the films and the cytotoxicity of the obtained copolymer samples were studied. The sample of a hybrid copolymer of collagen and PAA obtained at 60 °C has stronger mechanical properties compared to other tested samples. Its low cytotoxicity, when the monomer is removed, makes materials based on it promising in scaffold technologies.
在鱼胶原蛋白和丙烯酰胺（AA）的醋酸分散悬浮液中，于三丁基硼烷（TBB）存在的条件下合成了胶原蛋白与聚丙烯酰胺（PAA）的接枝共聚物。使用红外光谱和凝胶渗透色谱法（GPC）测定了共聚物的特性。25至60°C之间的合成温度差异对接枝聚丙烯酰胺的生成比例及其分子量均无显著影响。然而，借助扫描电子显微镜拍摄的显微照片显示，在合成温度高于25°C时，共聚物中的胶原蛋白纤维结构会遭到破坏。研究了薄膜的机械性能以及所得共聚物样品的细胞毒性。与其他测试样品相比，在60°C下获得的胶原蛋白与PAA杂化共聚物样品具有更强的机械性能。去除单体后，其低细胞毒性使基于该材料的产品在支架技术方面颇具前景。
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