School of Pharmaceutical Sciences, Jiangnan University, Wuxi, China.
Wuxi School of Medicine, Jiangnan University, Wuxi, China.
J Biomater Sci Polym Ed. 2020 Dec;31(17):2199-2219. doi: 10.1080/09205063.2020.1796229. Epub 2020 Jul 24.
Hydrogel scaffolds are explored as efficient methods to repair damaged organs or tissues. In this study, we developed a hybrid hydrogel system based on collagen (Col) and PEG-derived polymer (PEGF) for biomedical scaffold. The Col-PEGF hybrid hydrogel, in which different materials were combined and sequential interpenetrating networks were built, achieved significantly enhanced mechanical strength and viscoelasticity compared with the corresponding Col hydrogel or PEGF hydrogel. Degradation test indicated that Col enabled the hybrid hydrogel to be broken down enzymatic degradation while PEGF contributed to the anti-degradation of the hydrogel. This balanced biodegradability of Col-PEGF hydrogel would be advantageous to the application for tissue engineering and regenerative medicine. Moreover, the Col-PEGF hybrid hydrogel with micron-sized pores and variable moisture performed good biocompatibility to NIH-3T3 cells, and supplied a favorable environment for cell growth and proliferation. Therefore, the Col-PEGF hydrogel will provide a promising biomedical scaffold for the therapy of tissue defects.
水凝胶支架被探索作为修复受损器官或组织的有效方法。在这项研究中，我们开发了一种基于胶原（Col）和聚乙二醇衍生聚合物（PEGF）的混合水凝胶系统，用于生物医学支架。Col-PEGF 杂化水凝胶中不同材料的结合和顺序互穿网络的构建，使其与相应的 Col 水凝胶或 PEGF 水凝胶相比，显著提高了机械强度和粘弹性。降解试验表明，Col 使杂化水凝胶能够通过酶降解进行分解，而 PEGF 有助于水凝胶的抗降解。Col-PEGF 水凝胶的这种平衡的生物降解性将有利于组织工程和再生医学的应用。此外，具有微米级孔和可变水分的 Col-PEGF 杂化水凝胶对 NIH-3T3 细胞表现出良好的生物相容性，并为细胞生长和增殖提供了有利的环境。因此，Col-PEGF 水凝胶将为组织缺陷的治疗提供一种有前途的生物医学支架。
