Paul Maitry, Tanskanen Jaakko, Jääskeläinen Marko, Chang Wei, Dalal Ahan, Moshelion Menachem, Schulman Alan H
HiLIFE Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
Viikki Plant Science Centre (ViPS), University of Helsinki, Helsinki, Finland.
Front Plant Sci. 2023 Jun 12;14:1193284. doi: 10.3389/fpls.2023.1193284. eCollection 2023.
During drought, plants close their stomata at a critical soil water content (SWC), together with making diverse physiological, developmental, and biochemical responses.
Using precision-phenotyping lysimeters, we imposed pre-flowering drought on four barley varieties (Arvo, Golden Promise, Hankkija 673, and Morex) and followed their physiological responses. For Golden Promise, we carried out RNA-seq on leaf transcripts before and during drought and during recovery, also examining retrotransposon 1expression. Transcriptional data were subjected to network analysis.
The varieties differed by their critical SWC (ϴ), Hankkija 673 responding at the highest and Golden Promise at the lowest. Pathways connected to drought and salinity response were strongly upregulated during drought; pathways connected to growth and development were strongly downregulated. During recovery, growth and development pathways were upregulated; altogether, 117 networked genes involved in ubiquitin-mediated autophagy were downregulated.
The differential response to SWC suggests adaptation to distinct rainfall patterns. We identified several strongly differentially expressed genes not earlier associated with drought response in barley. transcription is strongly transcriptionally upregulated by drought and downregulated during recovery unequally between the investigated cultivars. The downregulation of networked autophagy genes suggests a role for autophagy in drought response; its importance to resilience should be further investigated.
在干旱期间，植物会在临界土壤含水量（SWC）时关闭气孔，并做出各种生理、发育和生化反应。
我们使用精确表型测定蒸渗仪，在四种大麦品种（Arvo、Golden Promise、Hankkija 673和Morex）的开花前施加干旱胁迫，并跟踪它们的生理反应。对于Golden Promise，我们在干旱前、干旱期间和恢复期间对叶片转录本进行了RNA测序，还检测了反转录转座子1的表达。对转录数据进行了网络分析。
这些品种的临界SWC（ϴ）不同，Hankkija 673的反应阈值最高，Golden Promise的最低。与干旱和盐度反应相关的途径在干旱期间强烈上调；与生长和发育相关的途径强烈下调。在恢复期间，生长和发育途径上调；共有117个参与泛素介导自噬的网络基因下调。
对SWC的差异反应表明适应不同的降雨模式。我们鉴定出了几个以前未与大麦干旱反应相关的强烈差异表达基因。干旱期间转录强烈上调，恢复期间在不同品种间下调程度不同。网络自噬基因的下调表明自噬在干旱反应中起作用；其对恢复力的重要性应进一步研究。
