Chen Silin, Li Ping, Tan Shunling, Pu Xiaojun, Zhou Ying, Hu Keming, Huang Wei, Liu Li
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Yunnan Key Laboratory for Wild Plant Resources, Department of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Plants (Basel). 2021 Jun 2;10(6):1127. doi: 10.3390/plants10061127.
Chloroplasts play essential roles in plant metabolic processes and stress responses by functioning as environmental sensors. Understanding chloroplast responses to drought stress and subsequent recovery will help the ability to improve stress tolerance in plants. Here, a combined proteomic and physiological approach was used to investigate the response mechanisms of chloroplasts to drought stress and subsequent recovery. Early in the stress response, changes in stomatal movement were accompanied by immediate changes in protein synthesis to sustain the photosynthetic process. Thereafter, increasing drought stress seriously affected photosynthetic efficiency and led to altered expression of photosynthesis- and carbon-fixation-related proteins to protect the plants against photo-oxidative damage. Additional repair mechanisms were activated at the early stage of recovery to restore physiological functions and repair drought-induced damages, even while the negative effects of drought stress were still ongoing. Prolonging the re-watering period led to the gradual recovery of photosynthesis at both physiological and protein levels, indicating that a long repair process is required to restore plant function. Our findings provide a precise view of drought and recovery response mechanisms in and serve as a reference for further investigation into the physiological and molecular mechanisms underlying plant drought tolerance.
叶绿体作为环境传感器，在植物代谢过程和应激反应中发挥着重要作用。了解叶绿体对干旱胁迫及后续恢复的反应，将有助于提高植物的胁迫耐受性。在此，采用蛋白质组学和生理学相结合的方法，研究叶绿体对干旱胁迫及后续恢复的反应机制。在胁迫反应早期，气孔运动的变化伴随着蛋白质合成的即时变化，以维持光合作用过程。此后，干旱胁迫加剧严重影响光合效率，并导致光合作用和碳固定相关蛋白的表达改变，以保护植物免受光氧化损伤。即使干旱胁迫的负面影响仍在持续，在恢复早期也会激活额外的修复机制，以恢复生理功能并修复干旱诱导的损伤。延长复水期导致光合作用在生理和蛋白质水平上逐渐恢复，这表明需要一个漫长的修复过程来恢复植物功能。我们的研究结果提供了叶绿体中干旱和恢复反应机制的精确视图，并为进一步研究植物耐旱性的生理和分子机制提供了参考。
