Chen Xinyi, Wang Hanle, Chen Hui, Ren Ling, Wang Wei, Xu Jingjie, Luo Chenqi, Hu Peike, Fu Qiuli, Yao Ke
Eye Center of the Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China.
iScience. 2023 May 19;26(6):106921. doi: 10.1016/j.isci.2023.106921. eCollection 2023 Jun 16.
Lens itself has limited regeneration functionality, thus we aimed to create regenerated lens with biological function to treat cataracts rather than the intraocular lens used in cataract surgery. We induced exogenous human embryonic stem cells to directionally differentiate into lens fate like cells , mixed these cells with hyaluronate, and then implanted the mixture into lens capsule to regenerate . We successfully achieved near-complete lens regeneration, and the thickness of the regenerated lens reached 85% of the contralateral eye, showing the characteristics of biconvex shape, transparency, and a thickness and diopter close to that of natural lenses. Meanwhile, the participation of Wnt/PCP pathway in lens regeneration was verified. The regenerated lens in this study was the most transparent, thickest, and most similar to the original natural lens that has thus far been reported. Overall, these findings offer a new therapeutic strategy for cataracts and other lens diseases.
晶状体自身的再生功能有限，因此我们旨在制造具有生物功能的再生晶状体来治疗白内障，而非用于白内障手术的人工晶状体。我们诱导外源性人类胚胎干细胞定向分化为晶状体样细胞，将这些细胞与透明质酸盐混合，然后将混合物植入晶状体囊内进行再生。我们成功实现了近乎完全的晶状体再生，再生晶状体的厚度达到对侧眼的85%，呈现出双凸形状、透明的特征，且厚度和屈光度接近天然晶状体。同时，验证了Wnt/PCP信号通路在晶状体再生中的参与情况。本研究中的再生晶状体是迄今为止报道的最透明、最厚且与原始天然晶状体最相似的。总体而言，这些发现为白内障及其他晶状体疾病提供了一种新的治疗策略。
