Krishtal Oleg
Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, 4 Bogomoletz street, 01024, Kyiv, Ukraine.
BBA Adv. 2023 Mar 16;3:100087. doi: 10.1016/j.bbadva.2023.100087. eCollection 2023.
The author recalls several particularly memorable events during his scientific career that led to the discovery of acid-sensing ion channels and ionotropic purinergic receptors. The readers learn of the events of 1975 when the first intracellular perfusion of the neuronal soma has been achieved- the event that led to the precise measurement of the calcium currents through the neuronal plasma membrane. Next, 1980 brings us to the functional discovery of the neuronal proton receptors found in mammalian sensory neurons. The molecular identity of these receptors was discovered in the lab of Dr. M. Lazdunsky and they were named acid-sensing ion channels or ASICs. Now it is clear that every mammalian neuron expresses at least one member of the ASICs family. And yet, ASICs are known for their functional diversity which is currently being studied extensively due to their prominence as pharmacological targets. Eventually, readers learn of the events of 1983 and the functional discovery of ionotropic purinergic receptors, and their molecular identification in the lab of Dr. R.A. North that coined the name of P2X ionotropic receptors.
作者回忆了其科学职业生涯中几个特别难忘的事件，这些事件促成了酸敏感离子通道和离子型嘌呤能受体的发现。读者了解到1975年首次实现对神经元胞体进行细胞内灌流的事件，该事件使得能够精确测量通过神经元质膜的钙电流。接下来，1980年让我们了解到在哺乳动物感觉神经元中发现的神经元质子受体的功能。这些受体的分子身份是在M. 拉兹敦斯基博士的实验室中发现的，它们被命名为酸敏感离子通道（ASICs）。现在很清楚，每个哺乳动物神经元至少表达ASICs家族的一个成员。然而，ASICs因其功能多样性而闻名，由于它们作为药理学靶点的突出地位，目前正在对其进行广泛研究。最后，读者了解到1983年的事件以及离子型嘌呤能受体的功能发现，以及它们在R.A. 诺思博士的实验室中的分子鉴定，该实验室创造了P2X离子型受体这个名称。
