Satpathy M M, Sharma N S, Kaur P, Arora A K
MVSc Student in Veterinary Microbiology, Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India.
Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India.
Iran J Vet Res. 2023;24(1):37-41. doi: 10.22099/IJVR.2023.43480.6365.
Antimicrobial resistance (AMR) is a burning issue in the present era. Mastitis in dairy animals is one of the most important causes of huge production loss to dairy farmers. The study aims to find the prevalence, antimicrobial resistance profile, and resistance genes in the extended-spectrum beta-lactamase-producing in mastitic milk.
A total of 125 milk samples were collected from Beetal goats suffering from clinical mastitis from different districts of Punjab and processed for bacterial isolation and further identification. The drug resistance profile of ESBL-producing and its associations with molecular markers was analyzed using statistical analysis.
The prevalence of ESBL-producing in dairy goats of Punjab was recorded as 6.4%. The isolates showed the highest resistance to the beta-lactam group of antibiotics. The resistance percentages of streptomycin, gentamicin, tetracycline, chloramphenicol, clotrimazole, and colistin were 50%, 37.5%, 50%, 25%, 25%, and 50%, respectively. The isolates showed intermediate resistance to imipenem (12.5%) and tetracycline (25%). The ESBL-producing isolates harbored the resistance genes (100%), (62.5%), (25%), (37.5%), (37.5%), (25%), (37.5%), (25%), (12.5%), (25%), and (50%). Tetracycline and sulphonamide resistances were statistically associated with their respective resistance genes (P<0.05). Streptomycin resistance was not statistically associated with the presence of the gene (P>0.05). The genes and were not recorded in any of the isolates. In this study, 12.5% of the isolates showed co-resistance to colistin and carbapenem.
Antimicrobial resistance is a hot topic and requires immediate attention.
抗菌药物耐药性（AMR）是当今时代的一个紧迫问题。奶牛乳腺炎是导致奶农巨大生产损失的最重要原因之一。本研究旨在查明患乳腺炎的牛奶中产超广谱β-内酰胺酶细菌的流行情况、抗菌药物耐药性概况及耐药基因。
从旁遮普不同地区患有临床乳腺炎的比etal山羊身上共采集125份牛奶样本，进行细菌分离及进一步鉴定。使用统计分析方法分析产ESBL细菌的耐药性概况及其与分子标记的关联。
旁遮普奶山羊中产ESBL细菌的流行率记录为6.4%。分离株对β-内酰胺类抗生素耐药性最高。链霉素、庆大霉素、四环素、氯霉素、克霉唑和黏菌素的耐药率分别为50%、37.5%、50%、25%、25%和50%。分离株对亚胺培南（12.5%）和四环素（25%）表现出中度耐药。产ESBL细菌分离株携带耐药基因blaTEM（100%）、blaSHV（62.5%）、blaCTX-M（25%）、tetA（37.5%）、tetB（37.5%）、sul1（25%）、sul2（37.5%）、strA（25%）、strB（12.5%）、aadA1（25%）和mcr-1（50%）。四环素和磺胺类耐药性与其各自的耐药基因在统计学上相关（P<0.05）。链霉素耐药性与blaSTR基因的存在在统计学上不相关（P>0.05）。在任何分离株中均未记录到blaNDM和blaOXA-48基因。在本研究中，12.5%的分离株对黏菌素和碳青霉烯类表现出共同耐药。
抗菌药物耐药性是一个热门话题，需要立即关注。
