El-Mohandes Shereen S, Eid Rasha H, Allam Ahmad M, Abou-Zeina Hala A A, Elbayoumy Mohamed K
Department of Mastitis and Neonatal Diseases, Animal Reproduction Research Institute, Agriculture Research Center, Giza 12622, Egypt.
Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, Giza 12556, Egypt.
Vet World. 2022 Apr;15(4):890-897. doi: 10.14202/vetworld.2022.890-897. Epub 2022 Apr 11.
Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have become a serious public health hazard worldwide. This importance is derived from the increase of new variants, particularly , , and genes. This study aimed to examine ESBL-producing isolated from different governorates in Egypt from dairy cows infected with subclinical and clinical mastitis.
This study examined 207 milk samples for the resistance of isolates against 14 different antibiotics and ran serological identification of ESBL-producing isolates with complete antibiotic resistance. Genotypic and sequencing analyses of several resistance genes were conducted using a polymerase chain reaction.
was identified in cases with subclinical mastitis (80.5%) and clinical mastitis (85.7%). ESBL-producing was isolated from 38.2% of subclinical mastitic milk compared to 39.3% in clinical cases, where O26:k60, O125:k70, and O25:k11 were the serotypes with complete resistance to antibiotics. ESBL-producing isolates were resistant to cefotaxime, amoxicillin, cloxacillin, oxacillin, rifampicin, and penicillin in 100% but susceptible to amoxicillin and clavulanic acid in 82.5% of the cases. Results also revealed that 51.25%, 52.5%, 66.25%, 77.5% and 60% of ESBL-producing isolates were responsive to ciprofloxacin, ofloxacin, norfloxacin, levofloxacin, and gentamycin, respectively. The detected genes were registered in GenBank as MW345819.1 and MW345820.1 for the gene and MW295407 for the gene.
This study found ESBL-producing in mastitic milk samples from Egyptian dairy farms and confirmed the occurrence and circulation of the main antibiotic genes ( and ) in the samples. Regular and thorough surveillance of ESBL-producing and subsequent preventive actions are essential for preventing the spread of these resistance genes in the future, which could pose serious and catastrophic health risks. Authorities should cling to the concept of One Health to minimize the risk of new varieties.
产超广谱β-内酰胺酶（ESBL）的肠杆菌科细菌已成为全球严重的公共卫生危害。这种重要性源于新变种的增加，特别是 、 和 基因。本研究旨在检测从埃及不同省份感染亚临床和临床乳腺炎的奶牛中分离出的产ESBL的 。
本研究检测了207份牛奶样本中分离株对14种不同抗生素的耐药性，并对产ESBL的 分离株进行了完全耐药的血清学鉴定。使用聚合酶链反应对几种耐药基因进行了基因型和测序分析。
在亚临床乳腺炎病例（80.5%）和临床乳腺炎病例（85.7%）中鉴定出 。在38.2%的亚临床乳腺炎乳汁中分离出产ESBL的 ，临床病例中这一比例为39.3%，其中O26:k60、O125:k70和O25:k11是对抗生素完全耐药的血清型。产ESBL的 分离株对头孢噻肟、阿莫西林、氯唑西林、苯唑西林、利福平和平霉素的耐药率为100%，但在82.5%的病例中对阿莫西林和克拉维酸敏感。结果还显示，产ESBL的 分离株对环丙沙星分别有51.25%、52.5%、66.25%、77.5%和60%的菌株对氧氟沙星、诺氟沙星、左氧氟沙星和庆大霉素有反应。检测到的基因在GenBank中注册为 基因MW345819.1和MW345820.1， 基因MW295407。
本研究在埃及奶牛场的乳腺炎乳汁样本中发现产ESBL的 ，并证实了样本中主要抗生素基因（ 和 ）的存在和传播。定期和彻底监测产ESBL的 并采取后续预防措施对于防止这些耐药基因在未来传播至关重要，因为这可能带来严重和灾难性的健康风险。当局应坚持“同一健康”理念，以降低新品种的风险。
