Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China.
Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, China.
Microbiol Spectr. 2023 Aug 17;11(4):e0536422. doi: 10.1128/spectrum.05364-22. Epub 2023 Jun 28.
In this study, we screened the gene cluster among 1,541 samples obtained from patients, healthy individuals, companion animals, pigs, chicken, and pork and chicken meat in Yangzhou, China. As a result, nine strains from humans, animals, and foods were positive for , which was located on plasmids or the chromosome. Seven different sequence types (STs) were identified, including ST15 ( = 2), ST580, ST1944, ST2294, ST5982, ST6262 ( = 2), and ST6265. All the positive strains were clustered into two distinct clades, and they shared a 24,087-bp core structure of , bounded by IS in the same orientation. IS could facilitate rapid and wide dissemination of in from various sources. Tigecycline has been regarded as one of the last-resort antibiotics available for the treatment of infections caused by carbapenem-resistant . The plasmid-mediated resistance-nodulation-division-type efflux pump gene cluster - is a newly identified tigecycline resistance determinant. In this study, we revealed that has disseminated among Klebsiella pneumoniae strains from poultry, food markets, and patients. It is critical to strengthen continuous monitoring, and control measures should be implemented to prevent the further dissemination of .
在这项研究中，我们在中国扬州的患者、健康个体、伴侣动物、猪、鸡和猪肉和鸡肉中筛选了 1541 个样本的基因簇。结果，有 9 株来自人类、动物和食物的菌株对 呈阳性，该基因位于质粒或染色体上。确定了 7 种不同的序列类型（ST），包括 ST15（=2）、ST580、ST1944、ST2294、ST5982、ST6262（=2）和 ST6265。所有阳性菌株分为两个不同的分支，它们共享一个 24087bp 的 核心结构，由相同方向的 IS 限定。IS 可以促进 从各种来源在 中快速广泛传播。替加环素被认为是治疗碳青霉烯类耐药 引起的感染的最后手段之一抗生素。质粒介导的耐药性结节分裂型外排泵基因簇 - 是新发现的替加环素耐药决定因素。在这项研究中，我们揭示了 已经在来自家禽、食品市场和患者的肺炎克雷伯菌菌株中传播。加强持续监测至关重要，应采取控制措施以防止 的进一步传播。
