State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
ACS Biomater Sci Eng. 2023 Jul 10;9(7):4197-4207. doi: 10.1021/acsbiomaterials.3c00217. Epub 2023 Jun 28.
There is an evident advantage in personalized customization of orthopedic implants by 3D-printed titanium (Ti) and its alloys. However, 3D-printed Ti alloys have a rough surface structure caused by adhesion powders and a relatively bioinert surface. Therefore, surface modification techniques are needed to improve the biocompatibility of 3D-printed Ti alloy implants. In the present study, porous Ti6Al4V scaffolds were manufactured by a selective laser melting 3D printer, followed by sandblasting and acid-etching treatment and atomic layer deposition (ALD) of tantalum oxide films. SEM morphology and surface roughness tests confirmed that the unmelted powders adhered on the scaffolds were removed by sandblasting and acid-etching. Accordingly, the porosity of the scaffold increased by about 7%. Benefiting from the self-limitation and three-dimensional conformance of ALD, uniform tantalum oxide films were formed on the inner and outer surfaces of the scaffolds. Zeta potential decreased by 19.5 mV after depositing tantalum oxide films. The results showed that the adhesion, proliferation, and osteogenic differentiation of rat bone marrow mesenchymal stem cells on modified Ti6Al4V scaffolds were significantly enhanced, which may be ascribed to surface structure optimization and the compatibility of tantalum oxide. This study provides a strategy to improve the cytocompatibility and osteogenic differentiation of porous Ti6Al4V scaffolds for orthopedic implants.
通过 3D 打印钛（Ti）及其合金对骨科植入物进行个性化定制具有明显优势。然而，3D 打印 Ti 合金由于附着粉末和相对生物惰性表面而具有粗糙的表面结构。因此，需要表面改性技术来提高 3D 打印 Ti 合金植入物的生物相容性。在本研究中，采用选择性激光熔化 3D 打印机制造多孔 Ti6Al4V 支架，然后进行喷砂和酸蚀处理以及原子层沉积（ALD）氧化钽薄膜。SEM 形貌和表面粗糙度测试证实，喷砂和酸蚀去除了附着在支架上的未熔化粉末。因此，支架的孔隙率增加了约 7%。得益于 ALD 的自限制和三维一致性，在支架的内外表面形成了均匀的氧化钽薄膜。沉积氧化钽薄膜后，Zeta 电位降低了 19.5 mV。结果表明，改性 Ti6Al4V 支架上大鼠骨髓间充质干细胞的黏附、增殖和成骨分化明显增强，这可能归因于表面结构优化和氧化钽的相容性。本研究为改善骨科植入物用多孔 Ti6Al4V 支架的细胞相容性和成骨分化提供了一种策略。
