Tasdemir Nur Kubra, Kilicarslan Bogac, Imren Gozde, Karaosmanoglu Beren, Taskiran Ekim Z, Bayram Cem
Department of Medical and Surgical Research, Hacettepe University, Institute of Health Sciences, Ankara, Turkey.
Department of Nanotechnology and Nanomedicine, Graduate School of Science and Engineering, Hacettepe University, Ankara, Turkey.
J R Soc Interface. 2025 Feb;22(223):20240642. doi: 10.1098/rsif.2024.0642. Epub 2025 Feb 26.
The concept of preconditioning mesenchymal stem cells (MSCs) under different stress conditions or with bioactive molecules is introduced to optimize their therapeutic potential. This study investigates the physicochemical effect of hierarchical TiO nanotube arrays, a versatile and easy-to-prepare nanosurface, on MSC behaviour. By precisely controlling the nanotopography through anodization, we demonstrate the significant influence of surface properties on MSC adhesion, proliferation and differentiation. Electrostatic interactions between surface charge and proteins play a crucial role in these cellular responses. In addition, preconditioning MSCs under specific conditions enhances their therapeutic potential by optimizing paracrine signalling and homing properties. Higher surface charges and increasing spiky character of surface roughness of titania samples after anodization at 60 V significantly upregulated chemokine receptor type 4 (CXCR4) and vascular endothelial growth factor A (VEGFA), indicating the enhanced migratory and angiogenic potential of MSCs. The study reveals the mechanotransductive effects of nanotopography on MSC differentiation, suggesting that tailored surface features can direct cellular fate. These findings highlight the potential of hierarchical TiO nanotube arrays as a promising platform for regenerative medicine, offering a novel approach to improve tissue engineering and therapeutic outcomes.
引入在不同应激条件下或使用生物活性分子对间充质干细胞（MSC）进行预处理的概念，以优化其治疗潜力。本研究调查了分层TiO纳米管阵列（一种通用且易于制备的纳米表面）对MSC行为的物理化学作用。通过阳极氧化精确控制纳米拓扑结构，我们证明了表面性质对MSC黏附、增殖和分化的显著影响。表面电荷与蛋白质之间的静电相互作用在这些细胞反应中起关键作用。此外，在特定条件下对MSC进行预处理可通过优化旁分泌信号传导和归巢特性来增强其治疗潜力。在60 V阳极氧化后，二氧化钛样品的更高表面电荷和表面粗糙度增加的尖刺特性显著上调了趋化因子受体4型（CXCR4）和血管内皮生长因子A（VEGFA），表明MSC的迁移和血管生成潜力增强。该研究揭示了纳米拓扑结构对MSC分化的机械转导作用，表明定制的表面特征可以引导细胞命运。这些发现突出了分层TiO纳米管阵列作为再生医学有前景平台的潜力，为改善组织工程和治疗效果提供了一种新方法。
