Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, China.
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China.
Plant Cell Rep. 2023 Sep;42(9):1517-1527. doi: 10.1007/s00299-023-03044-3. Epub 2023 Jun 28.
We analyzed the evolutionary pattern of cysteine-rich peptides (CRPs) to infer the relationship between CRP copy number and plant ecotype, and the origin of bi-domains CRPs. Plants produce cysteine-rich peptides (CRPs) that have long-lasting broad-spectrum antimicrobial activity to protect themselves from various groups of pathogens. We analyzed 240 plant genomes, ranging from algae to eudicots, and discovered that CRPs are widely distributed in plants. Our comparative genomics results revealed that CRP genes have been amplified through both whole genome and local tandem duplication. The copy number of these genes varied significantly across lineages and was associated with the plant ecotype. This may be due to their resistance to changing pathogenic environments. The conserved and lineage-specific CRP families contribute to diverse antimicrobial activities. Furthermore, we investigated the unique bi-domain CRPs that result from unequal crossover events. Our findings provide a unique evolutionary perspective on CRPs and insights into their antimicrobial and symbiosis characteristics.
我们分析了富含半胱氨酸的肽（CRPs）的进化模式，以推断 CRP 拷贝数与植物生态型之间的关系，以及双域 CRPs 的起源。植物产生富含半胱氨酸的肽（CRPs），以保护自己免受各种病原体的侵害。我们分析了 240 种植物基因组，从藻类到真双子叶植物，发现 CRPs 在植物中广泛分布。我们的比较基因组学结果表明，CRP 基因通过全基因组和局部串联重复扩增。这些基因的拷贝数在不同谱系中差异显著，并与植物生态型相关。这可能是由于它们对不断变化的病原环境的抵抗力。保守和谱系特异性 CRP 家族有助于多种抗菌活性。此外，我们研究了由不等交换事件产生的独特双域 CRPs。我们的研究结果为 CRPs 提供了独特的进化视角，并深入了解了它们的抗菌和共生特性。
