Environment Science and Engineering College, Yangzhou University, Yangzhou, Jiangsu 225009, China; Key Laboratory of Cultivated Land Quality Monitoring and Evaluation, Ministry of Agriculture and Rural Affairs, Yangzhou 225127, China; Jiangsu Collaborative Innovation Centre for Solid Organic Waste Resource Utilization, Nanjing, Jiangsu 210095, China.
Environment Science and Engineering College, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Ultrason Sonochem. 2023 Aug;98:106502. doi: 10.1016/j.ultsonch.2023.106502. Epub 2023 Jun 24.
1000 kHz high-frequency ultrasound at 0.12 and 0.39 W/mL intensity was used to enhance the inactivation of suspensions of Microcystis aeruginosa cells using KMnO. With 10 mg/L of KMnO, ultrasound at 0.12 W/mL intensity was found to be effective in inactivating the cyanobacteria within 10 min. A Weibull model was found to describes the inactivation well. Its concave shape shows that some cells have a certain resistance to this treatment. Cytometry and microscopic analysis confirm that the treatment damages cell integrity. Despite that the extracellular organic matter in the water was not significantly increased. The concentration of extracellular cyanobacterial toxins even decreased. The filtered suspension of inactivated cyanobacteria was used to cultivate mung beans, and the suspension did not hinder their germination. This provides a new idea for using cyanobacteria-laden wastewater. These findings suggest a technique for speeding up the oxidation of Microcystis cells using KMnO with ultrasound at moderate intensity, which provide new insights into the biological effects of ultrasound.
采用 1000kHz 高频超声，强度分别为 0.12 和 0.39W/mL，以增强 KMnO 对铜绿微囊藻细胞悬液的灭活效果。当 KMnO 浓度为 10mg/L 时，在 0.12W/mL 强度的超声作用下，10min 内可有效灭活蓝藻。Weibull 模型能很好地描述其灭活效果。模型的凹形表明，一些细胞对这种处理有一定的抗性。流式细胞术和显微镜分析证实，该处理破坏了细胞完整性。尽管水中的细胞外有机物没有明显增加，但细胞外蓝藻毒素的浓度甚至降低了。灭活蓝藻的过滤悬液用于培养绿豆，悬液并没有阻碍它们的发芽。这为利用富营养化废水提供了一个新的思路。这些发现为使用 KMnO 和中强度超声加速微囊藻细胞氧化提供了一种技术，为超声的生物学效应提供了新的见解。
